Effects of TAK-676 on immune cell populations within the tumor microenvironment in C57BL/6 mice bearing B16F10 syngeneic tumors (left) and BALB/c mice bearing CT26 syngeneic tumors (right). All data shown are representative of at least three independent experiments. A, Frequency of CD45⁺ cells in B16F10 tumors. B, Frequency of CD45⁺ cells in CT26 tumors. C, Frequency of CD8⁺ T cells in B16F10 tumors. D, Frequency of CD8⁺ T cells in CT26 tumors. E, Frequency of IFNγ⁺ CD8⁺ T cells in B16F10 tumors. F, MFI of Ki67 on CD8⁺ T cells in CT26 tumors. G, Frequency of CD8⁺ CD69⁺ T cells in B16F10 tumors. H, MFI of CD25 on CD8⁺ T cells in CT26 tumors. *, P ≤ 0.05; **, P ≤ 0.01. MHC, major histocompatibility complex.