Bisected and biantennary fucosylated structures are highly expressed in iCCA tumor. Representative images of the relative intensity of biantennary fucosylated N-glycan (1809.646 m/z); (A) bisected fucosylated N-glycan (2012.717 m/z); (B) tetraantennary branched N-glycan (2393.840 m/z); (C) high mannose N-glycan (1905.630 m/z); (D) proposed N-glycan structures at the bottom correspond to the respective m/z value (observed mass). (E) H&E staining. Tumor regions are outlined in red, normal areas are outlined in black, and fibrotic regions are outlined in blue. Intrahepatic Cholangiocarcinoma (iCCA), and Hepatocellular Carcinoma (HCC). For N-glycans, red triangle, fucose; blue square, N-acetylglucosamine; green circles, mannose; yellow circles, galactose.