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Table 4

E75 induced IFN-γ secretion in pre- and postvaccination patient PBMCs

Patient no.Vaccination statusStimulation group/IFN-γ (pg/ml)
NPaE75NPE75
−IL-12+IL-12
Post 1 0.0 0.0 0.0 0.0 
Post 1 0.0 0.0 0.0 65.0 
Post 1 0.0 0.0 0.0 106.3 
10 Pre 0.0 0.0 0.0 405.5 
 Post 1 0.0 108.0 479.7 782.2 
 Post 2b 176 211 194 276 
11 Pre 0.0 0.0 45.4 747.0 
 Post 1 0.0 0.0 56.5 840.1 
 Post 2 0.0 0.0 12.7 733.9 
14 Post 2 146 212 316 455 
Patient no.Vaccination statusStimulation group/IFN-γ (pg/ml)
NPaE75NPE75
−IL-12+IL-12
Post 1 0.0 0.0 0.0 0.0 
Post 1 0.0 0.0 0.0 65.0 
Post 1 0.0 0.0 0.0 106.3 
10 Pre 0.0 0.0 0.0 405.5 
 Post 1 0.0 108.0 479.7 782.2 
 Post 2b 176 211 194 276 
11 Pre 0.0 0.0 45.4 747.0 
 Post 1 0.0 0.0 56.5 840.1 
 Post 2 0.0 0.0 12.7 733.9 
14 Post 2 146 212 316 455 
a

No peptide control.

b

Supernatant collected after two rounds of stimulation; all determinations represent the highest values of IFN-γ in 24-h supernatants.

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