Quantitative values of pixel intensity per tumor in the Cy5.5 and Cy7 spectral range
| Mouse . | Regions of interest, average pixel intensity . | . | . | . | . | . | Signal-to-noise ratios . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Tumor subtype . | Tumor location . | Tumor Cy5.5 . | Tumor Cy7 . | Midline Cy5.5 . | Midline Cy7 . | Tumor/BG (Cy5.5) . | Tumor/BG (Cy7) . | ||||||
| 1 | 3T3/HER2+ | Right flank | 111.27 | 46.25 | 29.27 | 4.32 | 3.80 | 10.71 | ||||||
| 1 | 3T3/HER2+ | Left flank | 81.33 | 43.13 | 29.27 | 4.32 | 2.78 | 9.98 | ||||||
| 2 | 3T3/HER2+ | Right flank | 46.49 | 24.51 | 43.10 | 5.18 | 1.08 | 4.73 | ||||||
| 2 | 3T3/HER2+ | Left flank | 38.86 | 32.17 | 43.10 | 5.18 | 0.90 | 6.21 | ||||||
| 3 | 3T3/HER2+ | Right flank | 83.45 | 35.68 | 55.73 | 4.32 | 1.51 | 8.26 | ||||||
| 3 | 3T3/HER2+ | Left flank | 71.31 | 41.22 | 55.73 | 4.32 | 1.28 | 9.54 | ||||||
| 4 | 3T3/HER2+ | Right flank | 305.80 | 58.71 | 16.98 | 5.55 | 18.01 | 10.58 | ||||||
| 4 | 3T3/HER2+ | Left flank | 312.84 | 63.28 | 16.98 | 5.55 | 18.42 | 11.4 | ||||||
| 5 | 3T3/HER2+ | Right flank | 96.47 | 42.18 | 13.84 | 4.04 | 6.97 | 10.44 | ||||||
| 5 | 3T3/HER2+ | Left flank | 132.84 | 40.74 | 13.84 | 4.04 | 9.60 | 10.08 | ||||||
| 6 | A431 | Right flank | 349.95 | 5.03 | 15.83 | 5.14 | 22.11 | 0.98 | ||||||
| 6 | A431 | Left flank | 402.57 | 2.93 | 15.83 | 5.14 | 25.43 | 0.57 | ||||||
| 7 | A431 | Right flank | 404.92 | 12.45 | 24.28 | 5.65 | 16.68 | 2.20 | ||||||
| 7 | A431 | Left flank | 251.5 | 8.85 | 24.28 | 5.65 | 10.36 | 1.57 | ||||||
| 8 | A431 | Right flank | 224.78 | 9.8 | 27.11 | 5.80 | 8.29 | 1.69 | ||||||
| 8 | A431 | Left flank | 439.71 | 29.28 | 27.11 | 5.80 | 16.22 | 5.05 | ||||||
| 9 | A431 | Right flank | 410.81 | 27.69 | 29.68 | 6.58 | 13.84 | 4.21 | ||||||
| 9 | A431 | Left flank | 426.39 | 30.83 | 29.68 | 6.58 | 14.37 | 4.69 | ||||||
| 10 | A431 | Right flank | 350.24 | 12.09 | 24.80 | 5.54 | 14.12 | 2.18 | ||||||
| 10 | A431 | Left flank | 253.39 | 12.64 | 24.80 | 5.54 | 10.22 | 2.28 | ||||||
| Mouse . | Regions of interest, average pixel intensity . | . | . | . | . | . | Signal-to-noise ratios . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Tumor subtype . | Tumor location . | Tumor Cy5.5 . | Tumor Cy7 . | Midline Cy5.5 . | Midline Cy7 . | Tumor/BG (Cy5.5) . | Tumor/BG (Cy7) . | ||||||
| 1 | 3T3/HER2+ | Right flank | 111.27 | 46.25 | 29.27 | 4.32 | 3.80 | 10.71 | ||||||
| 1 | 3T3/HER2+ | Left flank | 81.33 | 43.13 | 29.27 | 4.32 | 2.78 | 9.98 | ||||||
| 2 | 3T3/HER2+ | Right flank | 46.49 | 24.51 | 43.10 | 5.18 | 1.08 | 4.73 | ||||||
| 2 | 3T3/HER2+ | Left flank | 38.86 | 32.17 | 43.10 | 5.18 | 0.90 | 6.21 | ||||||
| 3 | 3T3/HER2+ | Right flank | 83.45 | 35.68 | 55.73 | 4.32 | 1.51 | 8.26 | ||||||
| 3 | 3T3/HER2+ | Left flank | 71.31 | 41.22 | 55.73 | 4.32 | 1.28 | 9.54 | ||||||
| 4 | 3T3/HER2+ | Right flank | 305.80 | 58.71 | 16.98 | 5.55 | 18.01 | 10.58 | ||||||
| 4 | 3T3/HER2+ | Left flank | 312.84 | 63.28 | 16.98 | 5.55 | 18.42 | 11.4 | ||||||
| 5 | 3T3/HER2+ | Right flank | 96.47 | 42.18 | 13.84 | 4.04 | 6.97 | 10.44 | ||||||
| 5 | 3T3/HER2+ | Left flank | 132.84 | 40.74 | 13.84 | 4.04 | 9.60 | 10.08 | ||||||
| 6 | A431 | Right flank | 349.95 | 5.03 | 15.83 | 5.14 | 22.11 | 0.98 | ||||||
| 6 | A431 | Left flank | 402.57 | 2.93 | 15.83 | 5.14 | 25.43 | 0.57 | ||||||
| 7 | A431 | Right flank | 404.92 | 12.45 | 24.28 | 5.65 | 16.68 | 2.20 | ||||||
| 7 | A431 | Left flank | 251.5 | 8.85 | 24.28 | 5.65 | 10.36 | 1.57 | ||||||
| 8 | A431 | Right flank | 224.78 | 9.8 | 27.11 | 5.80 | 8.29 | 1.69 | ||||||
| 8 | A431 | Left flank | 439.71 | 29.28 | 27.11 | 5.80 | 16.22 | 5.05 | ||||||
| 9 | A431 | Right flank | 410.81 | 27.69 | 29.68 | 6.58 | 13.84 | 4.21 | ||||||
| 9 | A431 | Left flank | 426.39 | 30.83 | 29.68 | 6.58 | 14.37 | 4.69 | ||||||
| 10 | A431 | Right flank | 350.24 | 12.09 | 24.80 | 5.54 | 14.12 | 2.18 | ||||||
| 10 | A431 | Left flank | 253.39 | 12.64 | 24.80 | 5.54 | 10.22 | 2.28 | ||||||
NOTE: Background values were also obtained to allow the derivation of signal-to-noise ratios.