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Table 1.

Quantitative values of pixel intensity per tumor in the Cy5.5 and Cy7 spectral range

MouseRegions of interest, average pixel intensity
Signal-to-noise ratios
Tumor subtypeTumor locationTumor Cy5.5Tumor Cy7Midline Cy5.5Midline Cy7Tumor/BG (Cy5.5)Tumor/BG (Cy7)
3T3/HER2+ Right flank 111.27 46.25 29.27 4.32 3.80 10.71 
3T3/HER2+ Left flank 81.33 43.13 29.27 4.32 2.78 9.98 
3T3/HER2+ Right flank 46.49 24.51 43.10 5.18 1.08 4.73 
3T3/HER2+ Left flank 38.86 32.17 43.10 5.18 0.90 6.21 
3T3/HER2+ Right flank 83.45 35.68 55.73 4.32 1.51 8.26 
3T3/HER2+ Left flank 71.31 41.22 55.73 4.32 1.28 9.54 
3T3/HER2+ Right flank 305.80 58.71 16.98 5.55 18.01 10.58 
3T3/HER2+ Left flank 312.84 63.28 16.98 5.55 18.42 11.4 
3T3/HER2+ Right flank 96.47 42.18 13.84 4.04 6.97 10.44 
3T3/HER2+ Left flank 132.84 40.74 13.84 4.04 9.60 10.08 
A431 Right flank 349.95 5.03 15.83 5.14 22.11 0.98 
A431 Left flank 402.57 2.93 15.83 5.14 25.43 0.57 
A431 Right flank 404.92 12.45 24.28 5.65 16.68 2.20 
A431 Left flank 251.5 8.85 24.28 5.65 10.36 1.57 
A431 Right flank 224.78 9.8 27.11 5.80 8.29 1.69 
A431 Left flank 439.71 29.28 27.11 5.80 16.22 5.05 
A431 Right flank 410.81 27.69 29.68 6.58 13.84 4.21 
A431 Left flank 426.39 30.83 29.68 6.58 14.37 4.69 
10 A431 Right flank 350.24 12.09 24.80 5.54 14.12 2.18 
10 A431 Left flank 253.39 12.64 24.80 5.54 10.22 2.28 
MouseRegions of interest, average pixel intensity
Signal-to-noise ratios
Tumor subtypeTumor locationTumor Cy5.5Tumor Cy7Midline Cy5.5Midline Cy7Tumor/BG (Cy5.5)Tumor/BG (Cy7)
3T3/HER2+ Right flank 111.27 46.25 29.27 4.32 3.80 10.71 
3T3/HER2+ Left flank 81.33 43.13 29.27 4.32 2.78 9.98 
3T3/HER2+ Right flank 46.49 24.51 43.10 5.18 1.08 4.73 
3T3/HER2+ Left flank 38.86 32.17 43.10 5.18 0.90 6.21 
3T3/HER2+ Right flank 83.45 35.68 55.73 4.32 1.51 8.26 
3T3/HER2+ Left flank 71.31 41.22 55.73 4.32 1.28 9.54 
3T3/HER2+ Right flank 305.80 58.71 16.98 5.55 18.01 10.58 
3T3/HER2+ Left flank 312.84 63.28 16.98 5.55 18.42 11.4 
3T3/HER2+ Right flank 96.47 42.18 13.84 4.04 6.97 10.44 
3T3/HER2+ Left flank 132.84 40.74 13.84 4.04 9.60 10.08 
A431 Right flank 349.95 5.03 15.83 5.14 22.11 0.98 
A431 Left flank 402.57 2.93 15.83 5.14 25.43 0.57 
A431 Right flank 404.92 12.45 24.28 5.65 16.68 2.20 
A431 Left flank 251.5 8.85 24.28 5.65 10.36 1.57 
A431 Right flank 224.78 9.8 27.11 5.80 8.29 1.69 
A431 Left flank 439.71 29.28 27.11 5.80 16.22 5.05 
A431 Right flank 410.81 27.69 29.68 6.58 13.84 4.21 
A431 Left flank 426.39 30.83 29.68 6.58 14.37 4.69 
10 A431 Right flank 350.24 12.09 24.80 5.54 14.12 2.18 
10 A431 Left flank 253.39 12.64 24.80 5.54 10.22 2.28 

NOTE: Background values were also obtained to allow the derivation of signal-to-noise ratios.

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