Genes differentially expressed between samples of lung adenocarcinoma and mesothelioma and not expressed in normal mesothelial cells
| Gene (GenBank accession no.) . | Primers (5′ > 3′) . | Product size (bp) . | Affymetrix fold change (log2) . | Real-time fold change (log2) . |
|---|---|---|---|---|
| SFTP3 (NM_000542) | FWD: TTTGTGGAGCAGCACACG | 115 | 7.67 | 10.7 |
| REV: TACACTGGAGAGGGCTGGAC | ||||
| CEACAM6 (M18728) | FWD: ATGAAGAAGCAACCGGACAG | 97 | 6.64 | 10.4 |
| REV: ACAGCATCCTTGTCCTCCAC | ||||
| WFDC2 (X63187) | FWD: CCGACAACCTCAAGTGCTG | 105 | 4.61 | 3.59 |
| REV: CGAGCTGGGGAAAGTTAATG | ||||
| AGR2 (AF038451) | FWD: CGAACCTGCAGATACAGCTC | 105 | 4.48 | 7.40 |
| REV: GACAGACAGAAGGGCTTGGA | ||||
| CLDN7 (AJ011497) | FWD: CAAAATGTACGACTCGGTGCT | 100 | 4.07 | 4.33 |
| REV: CACAAACATGGCCAGGAAG | ||||
| CLDN3 (NM_001306) | FWD: AACATCATCACGTCGCAGAA | 98 | 4.07 | 5.33 |
| REV: AGCAGCGAGTCGTACACCTT | ||||
| ERBB3 (NM_001982) | FWD: GATGGGGAACCTTGAGATTG | 91 | 3.80 | 4.41 |
| REV: CACGAGGACATAGCCTGTCA | ||||
| FLJ12443 (AF052162) | FWD: CCTCGCACTTGTCGCATC | 96 | 3.16 | 2.32 |
| REV: ATGGCCTTCAGCAGGAAGT | ||||
| XMP (U52100) | FWD: CTGCAGCCTTGCTGTTCAT | 103 | 2.67 | 3.73 |
| REV: GTGCAATTCGTGTTGTTGGT | ||||
| LAD (U42408) | FWD: CTTCAGACCCTCCGACTGTG | 102 | 2.36 | 1.90 |
| REV: GCTGTGTGGGTGAGGACATA | ||||
| SPINT1 (BT007425) | FWD: CTCACGAGGGAAGTGTACCG | 93 | 1.67 | 1.59 |
| REV: GGTTCCTGGGGTTGTACCTT | ||||
| GAS6 (NM_000820) | FWD: CCTCTCTCTGTGGCACTGGT | 112 | −2.27 | −1.06 |
| REV: CGCAGACCTTGATCTCCATT | ||||
| SEMA3C (AB000220) | FWD: CAGCCCCAAGCAAGAGTTTA | 89 | −4.12 | −2.50 |
| REV: TCTAAAGGATGGTGGGAAAGG | ||||
| KIBRA (AB020676) | FWD: AAGCGGCTGGAAAAGGAC | 114 | −2.41 | −1.09 |
| REV: GGCTTCCTCCAGTTCTCTCA | ||||
| GFPT2 (NM_005110) | FWD: AGGCCGTGGAATTCTTCTTT | 110 | −3.47 | −2.86 |
| REV: GAGTTTCCCATCAGCCACTG | ||||
| S1-5 (EFEMP1) (NM_004105) | FWD: GCTAAGCAGTGACAGGCTCA | 93 | −4.12 | −3.37 |
| REV: TTCCCAGGTTCATTGACACA | ||||
| RALDH2 (AB015226) | FWD: GTAGTGGGGAGTCCCTTTGA | 97 | −4.53 | −5.75 |
| REV: CCACACCACTCTGGATGAGTT |
| Gene (GenBank accession no.) . | Primers (5′ > 3′) . | Product size (bp) . | Affymetrix fold change (log2) . | Real-time fold change (log2) . |
|---|---|---|---|---|
| SFTP3 (NM_000542) | FWD: TTTGTGGAGCAGCACACG | 115 | 7.67 | 10.7 |
| REV: TACACTGGAGAGGGCTGGAC | ||||
| CEACAM6 (M18728) | FWD: ATGAAGAAGCAACCGGACAG | 97 | 6.64 | 10.4 |
| REV: ACAGCATCCTTGTCCTCCAC | ||||
| WFDC2 (X63187) | FWD: CCGACAACCTCAAGTGCTG | 105 | 4.61 | 3.59 |
| REV: CGAGCTGGGGAAAGTTAATG | ||||
| AGR2 (AF038451) | FWD: CGAACCTGCAGATACAGCTC | 105 | 4.48 | 7.40 |
| REV: GACAGACAGAAGGGCTTGGA | ||||
| CLDN7 (AJ011497) | FWD: CAAAATGTACGACTCGGTGCT | 100 | 4.07 | 4.33 |
| REV: CACAAACATGGCCAGGAAG | ||||
| CLDN3 (NM_001306) | FWD: AACATCATCACGTCGCAGAA | 98 | 4.07 | 5.33 |
| REV: AGCAGCGAGTCGTACACCTT | ||||
| ERBB3 (NM_001982) | FWD: GATGGGGAACCTTGAGATTG | 91 | 3.80 | 4.41 |
| REV: CACGAGGACATAGCCTGTCA | ||||
| FLJ12443 (AF052162) | FWD: CCTCGCACTTGTCGCATC | 96 | 3.16 | 2.32 |
| REV: ATGGCCTTCAGCAGGAAGT | ||||
| XMP (U52100) | FWD: CTGCAGCCTTGCTGTTCAT | 103 | 2.67 | 3.73 |
| REV: GTGCAATTCGTGTTGTTGGT | ||||
| LAD (U42408) | FWD: CTTCAGACCCTCCGACTGTG | 102 | 2.36 | 1.90 |
| REV: GCTGTGTGGGTGAGGACATA | ||||
| SPINT1 (BT007425) | FWD: CTCACGAGGGAAGTGTACCG | 93 | 1.67 | 1.59 |
| REV: GGTTCCTGGGGTTGTACCTT | ||||
| GAS6 (NM_000820) | FWD: CCTCTCTCTGTGGCACTGGT | 112 | −2.27 | −1.06 |
| REV: CGCAGACCTTGATCTCCATT | ||||
| SEMA3C (AB000220) | FWD: CAGCCCCAAGCAAGAGTTTA | 89 | −4.12 | −2.50 |
| REV: TCTAAAGGATGGTGGGAAAGG | ||||
| KIBRA (AB020676) | FWD: AAGCGGCTGGAAAAGGAC | 114 | −2.41 | −1.09 |
| REV: GGCTTCCTCCAGTTCTCTCA | ||||
| GFPT2 (NM_005110) | FWD: AGGCCGTGGAATTCTTCTTT | 110 | −3.47 | −2.86 |
| REV: GAGTTTCCCATCAGCCACTG | ||||
| S1-5 (EFEMP1) (NM_004105) | FWD: GCTAAGCAGTGACAGGCTCA | 93 | −4.12 | −3.37 |
| REV: TTCCCAGGTTCATTGACACA | ||||
| RALDH2 (AB015226) | FWD: GTAGTGGGGAGTCCCTTTGA | 97 | −4.53 | −5.75 |
| REV: CCACACCACTCTGGATGAGTT |
NOTE: The primers used for quantitative RT-PCR analysis and the expected product sizes are shown. Log2-transformed fold change in median expression in lung adenocarcinoma relative to malignant mesothelioma for both Affymetrix U95A and quantitative RT-PCR are given. Gene expression levels were normalized relative to GAPDH probesets (Affymetrix data) or to quantitative RT-PCR controls.