Sequences of PCR primers and probes
| Cyclooxygenase-2 (COX-2) | ||
| Forward primer (COX2-633F) | 5′-GCTCAAACATGATGTTTGCATTC-3′ | |
| Reverse primer (COX2-711R) | 5′-GCTGGCCTCGCTTATGA-3′ | |
| Probe (COX2-658T) | 5′-(FAM)TGCCCAGCACTTCACGCATCAGTT(TAMRA)-3′ | |
| β-Actin | ||
| Forward primer (b-actin-592F) | 5′-TGAGCGGGCTACAGCTT-3′ | |
| Reverse primer (b-actin-651R) | 5′-TCCTTAATGTCACGCACGATTT-3′ | |
| Probe (b-actin-611T) | 5′-(FAM)ACCACCACGGCCGAGCGG(TAMRA)-3′ | |
| Cyclooxygenase-2 (COX-2) | ||
| Forward primer (COX2-633F) | 5′-GCTCAAACATGATGTTTGCATTC-3′ | |
| Reverse primer (COX2-711R) | 5′-GCTGGCCTCGCTTATGA-3′ | |
| Probe (COX2-658T) | 5′-(FAM)TGCCCAGCACTTCACGCATCAGTT(TAMRA)-3′ | |
| β-Actin | ||
| Forward primer (b-actin-592F) | 5′-TGAGCGGGCTACAGCTT-3′ | |
| Reverse primer (b-actin-651R) | 5′-TCCTTAATGTCACGCACGATTT-3′ | |
| Probe (b-actin-611T) | 5′-(FAM)ACCACCACGGCCGAGCGG(TAMRA)-3′ | |