Effect of the blocking procedures in the phage selection process
| Selection . | Panning . | Picking . | Tested clones . | Positive, soluble scFv . | Unique sequences . | Selected clones . |
|---|---|---|---|---|---|---|
| A1 | Third | Manual | 96 | 5 | 2 | 3A, 3B |
| A2 | Second | Picker | 96 | 3 | 1 | 3C |
| B | Third | Picker | 96 | 7 | 3 | 1D, 2D, 7D |
| Total | 288 | 15 | 6 |
| Selection . | Panning . | Picking . | Tested clones . | Positive, soluble scFv . | Unique sequences . | Selected clones . |
|---|---|---|---|---|---|---|
| A1 | Third | Manual | 96 | 5 | 2 | 3A, 3B |
| A2 | Second | Picker | 96 | 3 | 1 | 3C |
| B | Third | Picker | 96 | 7 | 3 | 1D, 2D, 7D |
| Total | 288 | 15 | 6 |