Expression analysis of CYP27B1, VDR, and CYP24 employing real-time PCR
| Origin . | Sample . | CYP27B1 . | . | VDR . | . | CYP24 . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | RQV . | RQV sample/RQV brain . | RQV . | RQV sample/RQV brain . | RQV . | RQV sample/RQV brain . | |||
| Biopsy | H91 (+)* | 2.1e−03 | 1.8e+00 | 2.1e−03 | 3.9e−02 | 2.0e−03 | 2.2e+01 | |||
| H220 (+) | 2.2e−03 | 1.9e+00 | 8.0e−04 | 1.5e−02 | 5.7e−03 | 6.4e+01 | ||||
| H246 (+)* | 4.7e−03 | 4.1e+00 | 3.7e−03 | 7.0e−02 | 1.9e−03 | 2.1e+01 | ||||
| H346 (+) | 3.1e−02 | 2.8e+01 | 1.9e−02 | 3.6e−01 | 0.0e+00 | 0.0e+00 | ||||
| H385 (+)* | 2.8e−03 | 2.4e+00 | 4.1e−03 | 7.7e−02 | 0.0e+00 | 0.0e+00 | ||||
| H549 (+) | 1.1e−02 | 9.8e+00 | 9.1e−03 | 1.7e−01 | 5.5e−04 | 6.1e+00 | ||||
| H552 (+) | 2.1e−01 | 1.8e+02 | 3.9e−03 | 7.4e−02 | 2.3e−04 | 2.5e+00 | ||||
| H556 (+) | 3.1e−03 | 2.7e+00 | 3.0e−03 | 5.6e−02 | 9.6e−05 | 1.1e+00 | ||||
| H570 (+) | 3.5e−03 | 3.1e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | ||||
| H981 (+) | 1.1e−02 | 9.9e+00 | 1.3e−02 | 2.5e−01 | 3.3e−03 | 3.6e+01 | ||||
| H49 (−) | 8.2e−03 | 7.2e+00 | 1.3e−03 | 2.5e−02 | 3.6e−03 | 4.1e+01 | ||||
| H226 (−) | 2.5e−02 | 2.2e+01 | 0.0e+00 | 0.0e+00 | 4.5e−04 | 5.0e+00 | ||||
| H281 (−) | 3.2e−02 | 2.9e+01 | 0.0e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | ||||
| H282 (−)* | 1.3e−01 | 1.1e+02 | 5.0e−03 | 9.5e−02 | 0.0e+00 | 0.0e+00 | ||||
| H321 (−) | 5.5e−05 | 4.8e−02 | 2.9e−04 | 5.5e−03 | 2.0e−03 | 2.3e+01 | ||||
| H323 (−) | 1.6e−03 | 1.4e+00 | 0.0e+00 | 0.0e+00 | 1.3e−02 | 1.5e+02 | ||||
| H361 (−)* | 6.1e−04 | 5.4e−01 | 2.0e−03 | 3.8e−02 | 0.0e+00 | 0.0e+00 | ||||
| H366 (−)* | 1.2e−03 | 1.1e+00 | 4.5e−01 | 8.5e+00 | 0.0e+00 | 0.0e+00 | ||||
| H545 (−) | 8.4e−04 | 7.4e−01 | 3.4e−03 | 6.5e−02 | 1.7e−02 | 1.9e+02 | ||||
| H546 (−) | 1.1e−03 | 9.6e−01 | 7.6e−04 | 1.4e−02 | 5.4e−05 | 6.0e−01 | ||||
| Cell line | T3868 (+) | 1.6e−02 | 1.5e+01 | 3.3e−03 | 6.3e−02 | 1.5e−04 | 1.7e+00 | |||
| TX3868 (+)* | 6.8e−02 | 6.0e+01 | 5.4e−03 | 1.0e−01 | 0.0e+00 | 0.0e+00 | ||||
| H246 (+) | 2.2e−02 | 1.9e+01 | 1.1e−03 | 2.2e−02 | 6.8e−03 | 7.6e+01 | ||||
| H346 (+) | 1.3e−02 | 1.1e+01 | 1.3e−04 | 2.5e−03 | 0.0e+00 | 0.0e+00 | ||||
| H556 (+) | 1.3e+00 | 1.2e+03 | 0.0e+00 | 0.0e+00 | 1.7e−05 | 1.9e−01 | ||||
| T3095 (−) | 2.9e−03 | 2.5e+00 | 1.3e−04 | 2.5e−03 | 0.0e+00 | 0.0e+00 | ||||
| TX3095 (−) | 4.7e−04 | 4.1e−01 | 3.5e−04 | 6.7e−03 | 0.0e+00 | 0.0e+00 | ||||
| H366 (−) | 2.5e−01 | 2.2e+02 | 0.0e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | ||||
| H545 (−) | 5.9e−02 | 5.2e+01 | 2.8e−03 | 5.3e−02 | 0.0e+00 | 0.0e+00 | ||||
| H546 (−) | 2.5e−02 | 2.2e+01 | 1.5e−02 | 2.8e−01 | 0.0e+00 | 0.0e+00 | ||||
| Biopsy | Brain | 1.1e−03 | 1 | 5.3e−02 | 1 | 9.0e−05 | 1 | |||
| Origin . | Sample . | CYP27B1 . | . | VDR . | . | CYP24 . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | RQV . | RQV sample/RQV brain . | RQV . | RQV sample/RQV brain . | RQV . | RQV sample/RQV brain . | |||
| Biopsy | H91 (+)* | 2.1e−03 | 1.8e+00 | 2.1e−03 | 3.9e−02 | 2.0e−03 | 2.2e+01 | |||
| H220 (+) | 2.2e−03 | 1.9e+00 | 8.0e−04 | 1.5e−02 | 5.7e−03 | 6.4e+01 | ||||
| H246 (+)* | 4.7e−03 | 4.1e+00 | 3.7e−03 | 7.0e−02 | 1.9e−03 | 2.1e+01 | ||||
| H346 (+) | 3.1e−02 | 2.8e+01 | 1.9e−02 | 3.6e−01 | 0.0e+00 | 0.0e+00 | ||||
| H385 (+)* | 2.8e−03 | 2.4e+00 | 4.1e−03 | 7.7e−02 | 0.0e+00 | 0.0e+00 | ||||
| H549 (+) | 1.1e−02 | 9.8e+00 | 9.1e−03 | 1.7e−01 | 5.5e−04 | 6.1e+00 | ||||
| H552 (+) | 2.1e−01 | 1.8e+02 | 3.9e−03 | 7.4e−02 | 2.3e−04 | 2.5e+00 | ||||
| H556 (+) | 3.1e−03 | 2.7e+00 | 3.0e−03 | 5.6e−02 | 9.6e−05 | 1.1e+00 | ||||
| H570 (+) | 3.5e−03 | 3.1e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | ||||
| H981 (+) | 1.1e−02 | 9.9e+00 | 1.3e−02 | 2.5e−01 | 3.3e−03 | 3.6e+01 | ||||
| H49 (−) | 8.2e−03 | 7.2e+00 | 1.3e−03 | 2.5e−02 | 3.6e−03 | 4.1e+01 | ||||
| H226 (−) | 2.5e−02 | 2.2e+01 | 0.0e+00 | 0.0e+00 | 4.5e−04 | 5.0e+00 | ||||
| H281 (−) | 3.2e−02 | 2.9e+01 | 0.0e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | ||||
| H282 (−)* | 1.3e−01 | 1.1e+02 | 5.0e−03 | 9.5e−02 | 0.0e+00 | 0.0e+00 | ||||
| H321 (−) | 5.5e−05 | 4.8e−02 | 2.9e−04 | 5.5e−03 | 2.0e−03 | 2.3e+01 | ||||
| H323 (−) | 1.6e−03 | 1.4e+00 | 0.0e+00 | 0.0e+00 | 1.3e−02 | 1.5e+02 | ||||
| H361 (−)* | 6.1e−04 | 5.4e−01 | 2.0e−03 | 3.8e−02 | 0.0e+00 | 0.0e+00 | ||||
| H366 (−)* | 1.2e−03 | 1.1e+00 | 4.5e−01 | 8.5e+00 | 0.0e+00 | 0.0e+00 | ||||
| H545 (−) | 8.4e−04 | 7.4e−01 | 3.4e−03 | 6.5e−02 | 1.7e−02 | 1.9e+02 | ||||
| H546 (−) | 1.1e−03 | 9.6e−01 | 7.6e−04 | 1.4e−02 | 5.4e−05 | 6.0e−01 | ||||
| Cell line | T3868 (+) | 1.6e−02 | 1.5e+01 | 3.3e−03 | 6.3e−02 | 1.5e−04 | 1.7e+00 | |||
| TX3868 (+)* | 6.8e−02 | 6.0e+01 | 5.4e−03 | 1.0e−01 | 0.0e+00 | 0.0e+00 | ||||
| H246 (+) | 2.2e−02 | 1.9e+01 | 1.1e−03 | 2.2e−02 | 6.8e−03 | 7.6e+01 | ||||
| H346 (+) | 1.3e−02 | 1.1e+01 | 1.3e−04 | 2.5e−03 | 0.0e+00 | 0.0e+00 | ||||
| H556 (+) | 1.3e+00 | 1.2e+03 | 0.0e+00 | 0.0e+00 | 1.7e−05 | 1.9e−01 | ||||
| T3095 (−) | 2.9e−03 | 2.5e+00 | 1.3e−04 | 2.5e−03 | 0.0e+00 | 0.0e+00 | ||||
| TX3095 (−) | 4.7e−04 | 4.1e−01 | 3.5e−04 | 6.7e−03 | 0.0e+00 | 0.0e+00 | ||||
| H366 (−) | 2.5e−01 | 2.2e+02 | 0.0e+00 | 0.0e+00 | 0.0e+00 | 0.0e+00 | ||||
| H545 (−) | 5.9e−02 | 5.2e+01 | 2.8e−03 | 5.3e−02 | 0.0e+00 | 0.0e+00 | ||||
| H546 (−) | 2.5e−02 | 2.2e+01 | 1.5e−02 | 2.8e−01 | 0.0e+00 | 0.0e+00 | ||||
| Biopsy | Brain | 1.1e−03 | 1 | 5.3e−02 | 1 | 9.0e−05 | 1 | |||
NOTE: Values provided represent means from duplicate assays from the same sample. (+), amplification of the CYP27B1 gene.(−), absence of amplification. RQV, relative quantification value.
CYP27B1 expression previously analyzed employing conventional RT-PCR (12).