Sequences of primers, probe, and calibration oligonucleotide
| Intron-spanning primers | Forward | Exon 2 | 5′-GCGTGGACAATGGCTACTCA-3′ |
| Reverse | Exon 3 | 5′-CCGCTTTTCTGTCTGGTTCC-3′ | |
| Dual-labelled fluorescent probe | Exon 3 | 5′-(FAMa)TGATTTGATGGAGTTGGACATGGCCA (TAMRAb)-3′ | |
| Oligonucleotide for calibration | 5′-ATCCAGCGTGGACAATGGCTACTCAAGCTGA TTTGATGGAGTTGGACATGGCCATGGAACCA GACAGAAAAGCGCTGTT-3′ |
| Intron-spanning primers | Forward | Exon 2 | 5′-GCGTGGACAATGGCTACTCA-3′ |
| Reverse | Exon 3 | 5′-CCGCTTTTCTGTCTGGTTCC-3′ | |
| Dual-labelled fluorescent probe | Exon 3 | 5′-(FAMa)TGATTTGATGGAGTTGGACATGGCCA (TAMRAb)-3′ | |
| Oligonucleotide for calibration | 5′-ATCCAGCGTGGACAATGGCTACTCAAGCTGA TTTGATGGAGTTGGACATGGCCATGGAACCA GACAGAAAAGCGCTGTT-3′ |