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Table 1.

Lapatinib induces G0-G1 arrest in the presence of IGF-I

SamplePercentage of cells per cycle phase
G0-G1SG2-M
Parental, serum-starved control 77.46 ± 0.29 8.8 ± 0.13 12.0 ± 0.05 
Parental, IGF-I + lapatinib 88.83 ± 0.06 1.6 ± 0.04 4.9 ± 0.12 
Pool 2, serum-starved control 66.87 ± 0.08 8.7 ± 0.31 21.68 ± 0.43 
Pool 2, IGF-I + lapatinib 80.41 ± 0.09 3.0 ± 0.03 13.33 ± 0.1 
Clone 3, serum-starved control 64.29 ± 0.34 13.55 ± 0.35 20.29 ± 0.08 
Clone 3, IGF-I + lapatinib 75.57 ± 4.43 2.22 ± 0.05 14.27 ± 0.45 
SamplePercentage of cells per cycle phase
G0-G1SG2-M
Parental, serum-starved control 77.46 ± 0.29 8.8 ± 0.13 12.0 ± 0.05 
Parental, IGF-I + lapatinib 88.83 ± 0.06 1.6 ± 0.04 4.9 ± 0.12 
Pool 2, serum-starved control 66.87 ± 0.08 8.7 ± 0.31 21.68 ± 0.43 
Pool 2, IGF-I + lapatinib 80.41 ± 0.09 3.0 ± 0.03 13.33 ± 0.1 
Clone 3, serum-starved control 64.29 ± 0.34 13.55 ± 0.35 20.29 ± 0.08 
Clone 3, IGF-I + lapatinib 75.57 ± 4.43 2.22 ± 0.05 14.27 ± 0.45 

NOTE: Parental, pool 2, and clone 3 cells were serum starved overnight and then either fixed without further treatment or incubated with IGF-I (100 ng/mL) and lapatinib (1 μmol/L) for 24 h before fixation. Cells were stained with propidium iodide, and DNA content was examined by flow cytometric cell cycle analysis (fluorescence-activated cell sorting). Percentages of cells in each cell cycle phase are shown with SD between duplicate cultures per group. Increased percentages of cells in G0-G1 and reduced percentages in S phase indicate that lapatinib induces cell cycle arrest at G0-G1 even in the presence of exogenously added IGF-I.

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