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Table 1.

DNA concentrations of wgaNTCs

WgaNTC*	DNA quantification method							Illumina genotyping
WgaNTC*		Quantitative PCR Alu Yd6 (ng/μL)	UV spectrophotometry (ng/μL)	Fluorescence λ curve (ng/μL)	Fluorescence calf thymus curve (ng/μL)	Sample call frequency^†
NTC 1	<0.008	42.3	26.6	52.9	0.969
NTC 2	<0.008	39.8	19.7	39.1	0.938
NTC 3	<0.008	34.5	19.5	40.0	0.940
NTC 4	<0.008	38.9	22.3	44.1	0.909

WgaNTC*	DNA quantification method							Illumina genotyping
WgaNTC*		Quantitative PCR Alu Yd6 (ng/μL)	UV spectrophotometry (ng/μL)	Fluorescence λ curve (ng/μL)	Fluorescence calf thymus curve (ng/μL)	Sample call frequency^†
NTC 1	<0.008	42.3	26.6	52.9	0.969
NTC 2	<0.008	39.8	19.7	39.1	0.938
NTC 3	<0.008	34.5	19.5	40.0	0.940
NTC 4	<0.008	38.9	22.3	44.1	0.909

NTC samples were WGA amplified with 10 μL of 1× Tris-EDTA in place of DNA alongside wgaDNA samples.

See Analysis.