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Table 1.

DNA concentrations of wgaNTCs

WgaNTC*DNA quantification method
Illumina genotyping
Quantitative PCR Alu Yd6 (ng/μL)UV spectrophotometry (ng/μL)Fluorescence λ curve (ng/μL)Fluorescence calf thymus curve (ng/μL)Sample call frequency
NTC 1 <0.008 42.3 26.6 52.9 0.969 
NTC 2 <0.008 39.8 19.7 39.1 0.938 
NTC 3 <0.008 34.5 19.5 40.0 0.940 
NTC 4 <0.008 38.9 22.3 44.1 0.909 
WgaNTC*DNA quantification method
Illumina genotyping
Quantitative PCR Alu Yd6 (ng/μL)UV spectrophotometry (ng/μL)Fluorescence λ curve (ng/μL)Fluorescence calf thymus curve (ng/μL)Sample call frequency
NTC 1 <0.008 42.3 26.6 52.9 0.969 
NTC 2 <0.008 39.8 19.7 39.1 0.938 
NTC 3 <0.008 34.5 19.5 40.0 0.940 
NTC 4 <0.008 38.9 22.3 44.1 0.909 
*

NTC samples were WGA amplified with 10 μL of 1× Tris-EDTA in place of DNA alongside wgaDNA samples.

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