Semiquantitative RT-PCR of WIBC-9 and non-IBC xenograftsa
| Target genes . | WIBC-9 . | MC-2 . | MC-5 . | MC- 18 . | SK- BR3 . | Activated lymphocyte . |
|---|---|---|---|---|---|---|
| EGFR family | ||||||
| EGFR | + | + | + | + | + | |
| ErbB-2 | + | − | − | − | + | |
| ErbB-3 | + | + | + | + | + | |
| ErbB-4 | + | + | + | + | + | |
| Angiogenic factors | ||||||
| VEGF | ++ | − | − | + | + | |
| VEGF-C | − | − | − | + | + | |
| VEGF-D | − | − | − | + | + | |
| ANG-1 | ++ | + | + | + | + | |
| ANG-2 | − | − | − | + | − | |
| bFGF | ++ | + | + | − | + | |
| Acid FGF | + | − | − | − | + | |
| TGF-α | + | − | − | − | + | |
| TGF-β1 | + | − | + | + | + | |
| VEGF receptors | ||||||
| flt-1 (mouse) | ++ | + | + | + | − | |
| flk-1 (mouse) | − | + | + | ++ | − | |
| flt-4 (mouse) | − | + | + | ++ | − | |
| Flt-1 | + | + | + | + | + | |
| KDR | + | + | + | + | + | |
| Angiopoietin receptors | ||||||
| tie-1 (mouse) | + | + | + | + | + | |
| tie-2 (mouse) | ++ | − | − | + | − | |
| Tie-1 | + | − | − | − | − | |
| Tie-2 | + | − | − | − | − | |
| Cytokines | ||||||
| IL-1β | + | − | − | − | − | − |
| IL-6 | − | − | − | − | − | + |
| IL-8 | ++ | + | + | + | + | + |
| IL-10 | − | − | − | − | − | + |
| IFN-γ | − | − | − | − | − | + |
| M-CSF | + | + | + | + | + | + |
| Adhesion molecules | ||||||
| Integrin αv | + | + | + | + | + | |
| Integrin αv (mouse) | ++ | + | ++ | + | − | |
| Integrin β3 | ++ | + | + | + | + | |
| Integrin β3 (mouse) | ++ | + | + | + | − | |
| Intercellular adhesion molecules | ||||||
| E-cadherin | + | + | + | + | + | |
| VE-cadherin (mouse) | − | + | + | + | − |
| Target genes . | WIBC-9 . | MC-2 . | MC-5 . | MC- 18 . | SK- BR3 . | Activated lymphocyte . |
|---|---|---|---|---|---|---|
| EGFR family | ||||||
| EGFR | + | + | + | + | + | |
| ErbB-2 | + | − | − | − | + | |
| ErbB-3 | + | + | + | + | + | |
| ErbB-4 | + | + | + | + | + | |
| Angiogenic factors | ||||||
| VEGF | ++ | − | − | + | + | |
| VEGF-C | − | − | − | + | + | |
| VEGF-D | − | − | − | + | + | |
| ANG-1 | ++ | + | + | + | + | |
| ANG-2 | − | − | − | + | − | |
| bFGF | ++ | + | + | − | + | |
| Acid FGF | + | − | − | − | + | |
| TGF-α | + | − | − | − | + | |
| TGF-β1 | + | − | + | + | + | |
| VEGF receptors | ||||||
| flt-1 (mouse) | ++ | + | + | + | − | |
| flk-1 (mouse) | − | + | + | ++ | − | |
| flt-4 (mouse) | − | + | + | ++ | − | |
| Flt-1 | + | + | + | + | + | |
| KDR | + | + | + | + | + | |
| Angiopoietin receptors | ||||||
| tie-1 (mouse) | + | + | + | + | + | |
| tie-2 (mouse) | ++ | − | − | + | − | |
| Tie-1 | + | − | − | − | − | |
| Tie-2 | + | − | − | − | − | |
| Cytokines | ||||||
| IL-1β | + | − | − | − | − | − |
| IL-6 | − | − | − | − | − | + |
| IL-8 | ++ | + | + | + | + | + |
| IL-10 | − | − | − | − | − | + |
| IFN-γ | − | − | − | − | − | + |
| M-CSF | + | + | + | + | + | + |
| Adhesion molecules | ||||||
| Integrin αv | + | + | + | + | + | |
| Integrin αv (mouse) | ++ | + | ++ | + | − | |
| Integrin β3 | ++ | + | + | + | + | |
| Integrin β3 (mouse) | ++ | + | + | + | − | |
| Intercellular adhesion molecules | ||||||
| E-cadherin | + | + | + | + | + | |
| VE-cadherin (mouse) | − | + | + | + | − |
Expression of genes for epidermal growth factors, angiogenic factors, cytokines, and EC adhesion molecules was assayed with RT-PCR. The relative expression levels were calculated as the density of the products divided by that of each internal control (− 0.3, 0.3 ≦ + < 1.0, ++≥ 1.0). M-CSF, macrophage colony-stimulating factor.