American Association for Cancer Research

Skip to Main Content

Table 2

Effect of NS-398 on three-dimensional culture systems

Growth assay
NS-398 (μm/ml)	Spheroid volume (mm³)
NS-398 (μm/ml)		U-87MG	U-251MG
Control(0)	29.85 ± 0.58	1.83 ± 0.13
50	25.35 ± 0.59^a	1.16 ± 0.32^a
100	15.53 ± 0.38^a	0.92 ± 0.33^a
150	8.43 ± 0.34^a	0.58 ± 0.45^a
200	6.07 ± 0.18^a	0.34 ± 0.20^a

Growth assay
NS-398 (μm/ml)	Spheroid volume (mm³)
NS-398 (μm/ml)		U-87MG	U-251MG
Control(0)	29.85 ± 0.58	1.83 ± 0.13
50	25.35 ± 0.59^a	1.16 ± 0.32^a
100	15.53 ± 0.38^a	0.92 ± 0.33^a
150	8.43 ± 0.34^a	0.58 ± 0.45^a
200	6.07 ± 0.18^a	0.34 ± 0.20^a

Migration assay
NS-398 (μm/ml)	Colony outgrowth area (mm²)
NS-398 (μm/ml)		U-87MG	U-251MG
Control(0)	15.99 ± 0.32	3.61 ± 0.19
50	12.67 ± 0.35^a	2.09 ± 0.90^a
100	9.96 ± 0.26^a	1.48 ± 0.94^a
150	6.46 ± 0.14^a	0.67 ± 0.36^a
200	4.51 ± 0.18^a	0.52 ± 0.03^a

Migration assay
NS-398 (μm/ml)	Colony outgrowth area (mm²)
NS-398 (μm/ml)		U-87MG	U-251MG
Control(0)	15.99 ± 0.32	3.61 ± 0.19
50	12.67 ± 0.35^a	2.09 ± 0.90^a
100	9.96 ± 0.26^a	1.48 ± 0.94^a
150	6.46 ± 0.14^a	0.67 ± 0.36^a
200	4.51 ± 0.18^a	0.52 ± 0.03^a

P < 0.0001.