American Association for Cancer Research

Table 1

Summary of spontaneous and PhIP-induced mutations at the HPRT locus in the HCT116 and DLD-1 cell lines^a

	DLD-1	DLD-1 + PhlP	HCT116	HCT116 + PhlP
Base substitutions^b	35 (88)	42 (95)	31 (69)	32 (76)
Transitions
G:C → A:T	14 (35)	17 (39)	21 (47)	15 (36)
A:T → G:C	5 (13)	1 (2)	2 (4)	3 (7)
Transversions
G:C → C:G	0	0	0	1 (2)
G:C → T:A	6 (15)	12 (27)	3 (7)	7 (17)
A:T → C:G	0	2 (5)	0	1 (2)
A:T → T:A	0	2 (5)	1 (2)	2 (5)
Putative splice^c	10 (25)	8 (18)	4 (9)	3 (7)
Frameshifts	5 (13)	2 (5)	14 (31)	10 (24)
Total number of mutants	40 (100)	44 (100)	45 (100)	42 (100)
Mutations in run of Gs^d	1 (3)	10 (23)	9 (20)	13 (31)
Transitions	0	4 (9)	0	3 (7)
Tranversions	1 (3)	4 (9)	0	2 (5)
+1 G	0	2 (5)	9 (20)	6 (14)
−1 G	0	0	0	2 (5)

	DLD-1	DLD-1 + PhlP	HCT116	HCT116 + PhlP
Base substitutions^b	35 (88)	42 (95)	31 (69)	32 (76)
Transitions
G:C → A:T	14 (35)	17 (39)	21 (47)	15 (36)
A:T → G:C	5 (13)	1 (2)	2 (4)	3 (7)
Transversions
G:C → C:G	0	0	0	1 (2)
G:C → T:A	6 (15)	12 (27)	3 (7)	7 (17)
A:T → C:G	0	2 (5)	0	1 (2)
A:T → T:A	0	2 (5)	1 (2)	2 (5)
Putative splice^c	10 (25)	8 (18)	4 (9)	3 (7)
Frameshifts	5 (13)	2 (5)	14 (31)	10 (24)
Total number of mutants	40 (100)	44 (100)	45 (100)	42 (100)
Mutations in run of Gs^d	1 (3)	10 (23)	9 (20)	13 (31)
Transitions	0	4 (9)	0	3 (7)
Tranversions	1 (3)	4 (9)	0	2 (5)
+1 G	0	2 (5)	9 (20)	6 (14)
−1 G	0	0	0	2 (5)

Incidence (percentage of total); spontaneous spectra reported previously (17).

Includes putative splice mutations as base substitutions.

Either exon deletions or deletion/insertion of several bases at exon junctions.

Run of six guanines, base 207 to 212 in exon 3.