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Table 1.

Immunophenotyping by mIF using two OPAL panels.

PhenotypeBiomarker expression
Opal Panel 1 
 Epithelial cells (CK+PD-L1+) + (CK+
 Epithelial cells PD-L1+ CK+PD-L1+ 
 Total T-cell lymphocytes (CD3+ only) + (CD3+CD8+PD1) + (CD3+PD1+) + (CD3+CD8+PD1+
 Cytotoxic T cells (CD3+CD8+PD1) + (CD3+CD8+PD1+
 T cells, antigen experienced (CD3+PD1+) + (CD3+CD8+PD1+
 Total macrophages (CD68+PD-L1) + (CD68+PD-L1+
 Macrophages PD-L1+ CD68+PD-L1+ 
Opal Panel 2 
 Total B-cell lymphocytes CD20+ 
 CD45RO+ cells CD45RO+ 
 Granzyme B+ cells Granzyme B+ 
 FOXP3+ cells FOXP3+ 
 CD57+ cells CD57+ 
PhenotypeBiomarker expression
Opal Panel 1 
 Epithelial cells (CK+PD-L1+) + (CK+
 Epithelial cells PD-L1+ CK+PD-L1+ 
 Total T-cell lymphocytes (CD3+ only) + (CD3+CD8+PD1) + (CD3+PD1+) + (CD3+CD8+PD1+
 Cytotoxic T cells (CD3+CD8+PD1) + (CD3+CD8+PD1+
 T cells, antigen experienced (CD3+PD1+) + (CD3+CD8+PD1+
 Total macrophages (CD68+PD-L1) + (CD68+PD-L1+
 Macrophages PD-L1+ CD68+PD-L1+ 
Opal Panel 2 
 Total B-cell lymphocytes CD20+ 
 CD45RO+ cells CD45RO+ 
 Granzyme B+ cells Granzyme B+ 
 FOXP3+ cells FOXP3+ 
 CD57+ cells CD57+ 
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