Biodistribution of RCR vector after locoregional virus infusion in immunocompetent CT26 hepatic metastasis model
| Copy number of RCR vector in vivo . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|
| Vector . | Prodrug . | Number of vector copies per 5 × 104 cells . | . | . | ||||
| . | . | Tumor . | Liver . | Bone marrow . | ||||
| No vector | Untreated | — | — | — | ||||
| No vector | Untreated | — | — | — | ||||
| ACE-CD | PBS | 16,623 | — | — | ||||
| ACE-CD | PBS | 11,223 | — | — | ||||
| ACE-CD | 5FC | 8,066 | — | — | ||||
| ACE-CD | 5FC | 11,174 | — | — | ||||
| Copy number of RCR vector in vivo . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|
| Vector . | Prodrug . | Number of vector copies per 5 × 104 cells . | . | . | ||||
| . | . | Tumor . | Liver . | Bone marrow . | ||||
| No vector | Untreated | — | — | — | ||||
| No vector | Untreated | — | — | — | ||||
| ACE-CD | PBS | 16,623 | — | — | ||||
| ACE-CD | PBS | 11,223 | — | — | ||||
| ACE-CD | 5FC | 8,066 | — | — | ||||
| ACE-CD | 5FC | 11,174 | — | — | ||||
NOTE: Genomic DNA, extracted from liver tumor, normal liver, or bone marrow of ACE-GFP infected mice, was analyzed by quantitative real-time PCR as described in Materials and Methods. As an internal control, each sample was also amplified with mouse β-actin specific primers.
Abbreviation: —, not detectable (detection limit was 20 copies per 5 × 104 cells).