Effect of background/age, Nbs1 genotype, ethylnitrosourea treatment, and Helicobacter status on tumor multiplicity and progression
| Mouse type . | n . | Lifespan (d), mean ± SD . | Intestinal tumor count, mean ± SD . | Invasive adenocarcinomas/total tumors (%)* . | ||||
|---|---|---|---|---|---|---|---|---|
| (A) Phenotype of F1 and controls | ||||||||
| (BR × B6)F1 | 72 | 232 ± 66 | 19 ± 10 | 104/185 (56) | ||||
| (SWR × B6)F1 | 79 | 370 ± 99 | 15 ± 10 | 39/45 (87) | ||||
| B6 | 97 | 100 ± 18 | 122 ± 45 | 0/74 (0) | ||||
| (B) Effect of Nbs1 | ||||||||
| B6 Nbs1+/+ | 17 | 144 ± 48 | 23 ± 23† | 6/53 (11) | ||||
| B6 Nbs1+/ΔB | 28 | 175 ± 79 | 24 ± 14† | ND | ||||
| B6 Nbs1 ΔB/ ΔB | 22 | 142 ± 42 | 31 ± 27† | 10/47 (21) | ||||
| B6 Apc+/+ Nbs1 ΔB/ΔB | 13 | ND | 0 ± 0 | NA | ||||
| (C) Effect of ethylnitrosourea treatment | ||||||||
| (BR × B6)F1, one ethylnitrosourea treatment | 9 | 202 ± 47 | 44 ± 22 | 8/94 (9)‡ | ||||
| (BR × B6)F1, two ethylnitrosourea treatments | 6 | 180 ± 8 | 58 ± 20 | |||||
| (SWR × B6)F1, one ethylnitrosourea treatment | 5 | 289 ± 86 | 19 ± 14 | 13/35 (38)‡ | ||||
| (SWR × B6)F1, two ethylnitrosourea treatments | 8 | 190 ± 21 | 40 ± 17 | |||||
| (D) Effect of Helicobacter | ||||||||
| (BR × B6)F1, Helicobacter negative | 61 | 312 ± 82 | 10 ± 4 | 9/30 (30) | ||||
| (BR × B6)F1, Helicobacter positive | 20 | 319 ± 100 | 12 ± 7 | 13/41 (32) | ||||
| (SWR × B6)F1, Helicobacter negative | 58 | 396 ± 144 | 13 ± 10 | 12/38 (32)§ | ||||
| (SWR × B6)F1, Helicobacter positive | 12 | 498 ± 75 | 8 ± 4 | 7/29 (24)§ | ||||
| Mouse type . | n . | Lifespan (d), mean ± SD . | Intestinal tumor count, mean ± SD . | Invasive adenocarcinomas/total tumors (%)* . | ||||
|---|---|---|---|---|---|---|---|---|
| (A) Phenotype of F1 and controls | ||||||||
| (BR × B6)F1 | 72 | 232 ± 66 | 19 ± 10 | 104/185 (56) | ||||
| (SWR × B6)F1 | 79 | 370 ± 99 | 15 ± 10 | 39/45 (87) | ||||
| B6 | 97 | 100 ± 18 | 122 ± 45 | 0/74 (0) | ||||
| (B) Effect of Nbs1 | ||||||||
| B6 Nbs1+/+ | 17 | 144 ± 48 | 23 ± 23† | 6/53 (11) | ||||
| B6 Nbs1+/ΔB | 28 | 175 ± 79 | 24 ± 14† | ND | ||||
| B6 Nbs1 ΔB/ ΔB | 22 | 142 ± 42 | 31 ± 27† | 10/47 (21) | ||||
| B6 Apc+/+ Nbs1 ΔB/ΔB | 13 | ND | 0 ± 0 | NA | ||||
| (C) Effect of ethylnitrosourea treatment | ||||||||
| (BR × B6)F1, one ethylnitrosourea treatment | 9 | 202 ± 47 | 44 ± 22 | 8/94 (9)‡ | ||||
| (BR × B6)F1, two ethylnitrosourea treatments | 6 | 180 ± 8 | 58 ± 20 | |||||
| (SWR × B6)F1, one ethylnitrosourea treatment | 5 | 289 ± 86 | 19 ± 14 | 13/35 (38)‡ | ||||
| (SWR × B6)F1, two ethylnitrosourea treatments | 8 | 190 ± 21 | 40 ± 17 | |||||
| (D) Effect of Helicobacter | ||||||||
| (BR × B6)F1, Helicobacter negative | 61 | 312 ± 82 | 10 ± 4 | 9/30 (30) | ||||
| (BR × B6)F1, Helicobacter positive | 20 | 319 ± 100 | 12 ± 7 | 13/41 (32) | ||||
| (SWR × B6)F1, Helicobacter negative | 58 | 396 ± 144 | 13 ± 10 | 12/38 (32)§ | ||||
| (SWR × B6)F1, Helicobacter positive | 12 | 498 ± 75 | 8 ± 4 | 7/29 (24)§ | ||||
NOTE: All mice are ApcMin/+ unless otherwise specified.
Intestinal tumors were isolated from a randomly selected subset of mice of each strain.
Wilcoxon rank-sum P values of the three pair-wise comparisons ≥ 0.3. ND, not done; NA, not applicable.
One or two ethylnitrosourea treatments.
One tumor in this set showed metastasis by direct extension to a regional lymph node.