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Table 3.

Response of transduced IMR-32 and IMR/KAT100 cells to cytotoxic drugs in 72-h cytotoxicity assays

DrugCell lineFold resistance*PCell lineFold resistance*PCell lineFold sensitizationP§
Doxorubicin IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 2.46 0.045 IMR/shp53 3.10 0.004 IMR/KAT100/shp53 0.57 NS 
Vincristine IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 1.64 0.039 IMR/shp53 3.62 0.036 IMR/KAT100/shp53 0.85 NS 
VM26 IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 1.63 0.013 IMR/shp53 1.51 0.002 IMR/KAT100/shp53 0.13 NS 
Cisplatin IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 3.92 0.001 IMR/shp53 3.01 0.004 IMR/KAT100/shp53 0.45 NS 
KAT IMR/LXSP   IMR/shGFP      
 IMR/GSE56 1.29 0.024 IMR/shp53 1.31 0.024    
Melphalan IMR/LXSP   IMR/shGFP      
 IMR/GSE56 20.19 <0.001 IMR/shp53 — —    
Taxol IMR/LXSP   IMR/shGFP      
 IMR/GSE56 2.29 NS IMR/shp53 1.72 NS    
DrugCell lineFold resistance*PCell lineFold resistance*PCell lineFold sensitizationP§
Doxorubicin IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 2.46 0.045 IMR/shp53 3.10 0.004 IMR/KAT100/shp53 0.57 NS 
Vincristine IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 1.64 0.039 IMR/shp53 3.62 0.036 IMR/KAT100/shp53 0.85 NS 
VM26 IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 1.63 0.013 IMR/shp53 1.51 0.002 IMR/KAT100/shp53 0.13 NS 
Cisplatin IMR/LXSP   IMR/shGFP   IMR/KAT100/shGFP   
 IMR/GSE56 3.92 0.001 IMR/shp53 3.01 0.004 IMR/KAT100/shp53 0.45 NS 
KAT IMR/LXSP   IMR/shGFP      
 IMR/GSE56 1.29 0.024 IMR/shp53 1.31 0.024    
Melphalan IMR/LXSP   IMR/shGFP      
 IMR/GSE56 20.19 <0.001 IMR/shp53 — —    
Taxol IMR/LXSP   IMR/shGFP      
 IMR/GSE56 2.29 NS IMR/shp53 1.72 NS    
*

The fold resistance was determined by dividing the ID50 of IMR/GSE56 or IMR/shp53 cells by that of control IMR/LXSP or IMR/shGFP cells, respectively.

For a given drug, P values were determined by comparing the ID50 value for IMR/GSE56 or IMR/shp53 cells with the ID50 value of their corresponding control cells assayed in the same series of experiments.

The fold sensitization was calculated by dividing the mean ID50 for the control IMR/KAT100/shGFP cells by the mean ID50 for the IMR/KAT100/shp53 cells.

§

For a given drug, P valves were determined by comparing the ID50 value for IMR/KAT100/shGFP cells with the ID50 value of IMR/KAT100/shp53 cells assayed in the same series of experiments.

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