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Table 1.

Response of p53 M135 SH-EP transfectants to cytotoxic drugs in 72-h cytotoxicity assays

DrugCell lineID50Fold resistance*P
KAT SHEP/CMV 6.731 ± 1.115 μg/mL    
 SHEP/M135 15.943 ± 1.834 μg/mL  2.37 0.023 
      

 

 
Log (drug) ± SE
 
M§
 

 

 
Doxorubicin SHEP/CMV −7.690 ± 0.050 2.042 × 10−8   
 SHEP/M135 −7.119 ± 0.056 7.603 × 10−8 3.72 <0.001 
Vincristine SHEP/CMV −9.163 ± 0.028 6.87 × 10−10   
 SHEP/M135 −8.550 ± 0.035 2.82 × 10−9 4.10 <0.001 
VM26 SHEP/CMV −6.987 ± 0.083 1.03 × 10−7   
 SHEP/M135 −5.928 ± 0.107 1.18 × 10−6 11.46 <0.001 
Cisplatin SHEP/CMV −5.851 ± 0.030 1.41 × 10−6   
 SHEP/M135 −5.517 ± 0.037 3.04 × 10−6 2.16 <0.001 
Methotrexate SHEP/CMV −7.834 ± 0.092 1.47 × 10−8   
 SHEP/M135 −7.564 ± 0.101 2.73 × 10−8 1.86 NS 
Taxol SHEP/CMV −8.261 ± 0.061 5.48 × 10−9   
 SHEP/M135 −8.166 ± 0.046 6.82 × 10−9 1.24 NS 
DrugCell lineID50Fold resistance*P
KAT SHEP/CMV 6.731 ± 1.115 μg/mL    
 SHEP/M135 15.943 ± 1.834 μg/mL  2.37 0.023 
      

 

 
Log (drug) ± SE
 
M§
 

 

 
Doxorubicin SHEP/CMV −7.690 ± 0.050 2.042 × 10−8   
 SHEP/M135 −7.119 ± 0.056 7.603 × 10−8 3.72 <0.001 
Vincristine SHEP/CMV −9.163 ± 0.028 6.87 × 10−10   
 SHEP/M135 −8.550 ± 0.035 2.82 × 10−9 4.10 <0.001 
VM26 SHEP/CMV −6.987 ± 0.083 1.03 × 10−7   
 SHEP/M135 −5.928 ± 0.107 1.18 × 10−6 11.46 <0.001 
Cisplatin SHEP/CMV −5.851 ± 0.030 1.41 × 10−6   
 SHEP/M135 −5.517 ± 0.037 3.04 × 10−6 2.16 <0.001 
Methotrexate SHEP/CMV −7.834 ± 0.092 1.47 × 10−8   
 SHEP/M135 −7.564 ± 0.101 2.73 × 10−8 1.86 NS 
Taxol SHEP/CMV −8.261 ± 0.061 5.48 × 10−9   
 SHEP/M135 −8.166 ± 0.046 6.82 × 10−9 1.24 NS 

Abbreviation: NS, not significant.

*

The fold resistance was determined by dividing the ID50 of the p53 M135–transfected SH-EP cells by that of the vector control SH-EP/CMV cells.

For a given drug, P values were determined by comparing the ID50 value for the p53 M135–transfected SH-EP cells with the ID50 value of the control SH-EP/CMV cells assayed in the same series of experiments.

Calculated from at least three replicate assays.

§

Molar concentration.

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