Virus replication in LN229, D247MG, and NHA cells under normoxia (N) versus hypoxia (Hyp) following infection at MOI 1, 5, and 1, respectively
| . | Cell lysate . | . | . | Conditioned media . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | N (×105) . | Hyp (×105) . | Fold change . | N (×103) . | Hyp (×103) . | Fold change . | ||||||
| LN229 day 3 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 376 ± 77 | 324 ± 66 | 1 | 513 ± 46 | 728 ± 133 | 1 | ||||||
| HYPR-Ad#1 | 4 ± 1 | 25 ± 6 | 6* | 2 ± 1 | 7 ± 3 | 4 | ||||||
| HYPR-Ad-mIL4 | 0.4 ± 0.1 | 20 ± 4 | 50* | 1 ± 0.7 | 2 ± 1 | 2 | ||||||
| LN229 day 6 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 3,192 ± 793 | 1,060 ± 310 | −3* | 141,181 ± 12,546 | 79,743 ± 9,406 | −2* | ||||||
| HYPR-Ad#1 | 10 ± 1 | 31 ± 6 | 3* | 6 ± 3 | 95 ± 30 | 16* | ||||||
| HYPR-Ad-mIL4 | 1 ± 0.3 | 23 ± 5 | 23* | 3 ± 3 | 83 ± 14 | 28* | ||||||
| NHA day 3 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 1,392 ± 175 | 1,538 ± 266 | 1 | 859 ± 159 | 696 ± 144 | 0.8 | ||||||
| HYPR-Ad#1 | 57 ± 5 | 282 ± 44 | 5* | 1.4 ± 1.0 | 9.5 ± 1.6 | 6.7* | ||||||
| HYPR-Ad-mIL4 | 18 ± 3 | 319 ± 47 | 23* | 0.0 ± 0.4 | 10.8 ± 3.9 | 12.0* | ||||||
| D247MG day 3 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 228 ± 73 | 452 ± 101 | 2* | 69 ± 17 | 132 ± 22 | 1.9 | ||||||
| HYPR-Ad#1 | 10 ± 4 | 102 ± 15 | 10* | 2 ± 0.3 | 9 ± 0.4 | 4.5* | ||||||
| HYPR-Ad-mIL4 | 5 ± 2 | 101 ± 16 | 20* | 2 ± 0.5 | 13 ± 0.4 | 6.5* | ||||||
| . | Cell lysate . | . | . | Conditioned media . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | N (×105) . | Hyp (×105) . | Fold change . | N (×103) . | Hyp (×103) . | Fold change . | ||||||
| LN229 day 3 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 376 ± 77 | 324 ± 66 | 1 | 513 ± 46 | 728 ± 133 | 1 | ||||||
| HYPR-Ad#1 | 4 ± 1 | 25 ± 6 | 6* | 2 ± 1 | 7 ± 3 | 4 | ||||||
| HYPR-Ad-mIL4 | 0.4 ± 0.1 | 20 ± 4 | 50* | 1 ± 0.7 | 2 ± 1 | 2 | ||||||
| LN229 day 6 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 3,192 ± 793 | 1,060 ± 310 | −3* | 141,181 ± 12,546 | 79,743 ± 9,406 | −2* | ||||||
| HYPR-Ad#1 | 10 ± 1 | 31 ± 6 | 3* | 6 ± 3 | 95 ± 30 | 16* | ||||||
| HYPR-Ad-mIL4 | 1 ± 0.3 | 23 ± 5 | 23* | 3 ± 3 | 83 ± 14 | 28* | ||||||
| NHA day 3 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 1,392 ± 175 | 1,538 ± 266 | 1 | 859 ± 159 | 696 ± 144 | 0.8 | ||||||
| HYPR-Ad#1 | 57 ± 5 | 282 ± 44 | 5* | 1.4 ± 1.0 | 9.5 ± 1.6 | 6.7* | ||||||
| HYPR-Ad-mIL4 | 18 ± 3 | 319 ± 47 | 23* | 0.0 ± 0.4 | 10.8 ± 3.9 | 12.0* | ||||||
| D247MG day 3 | ||||||||||||
| AdLacZ | 0 ± 0 | 0 ± 0 | 0 | 0 ± 0 | 0 ± 0 | 0 | ||||||
| dl309-Ad | 228 ± 73 | 452 ± 101 | 2* | 69 ± 17 | 132 ± 22 | 1.9 | ||||||
| HYPR-Ad#1 | 10 ± 4 | 102 ± 15 | 10* | 2 ± 0.3 | 9 ± 0.4 | 4.5* | ||||||
| HYPR-Ad-mIL4 | 5 ± 2 | 101 ± 16 | 20* | 2 ± 0.5 | 13 ± 0.4 | 6.5* | ||||||
NOTE: The data represent the mean IFU/mL ± SD.
Statistically significant (Student's t test) increase or decrease in virus production under hypoxia (P < 0.0118).