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Table 2.

Abundance of eicosanoid pathway enzymes in PC-3 cells

Relative abundance
Fold increase
ControlAA
COX-2 14.59 ± 7.94 275.83 ± 8.14 22.44 
COX-1 4.82 ± 0.40 7.43 ± 1.31 1.55 
5-LO 16.09 ± 5.58 20.03 ± 7.03 NS 
Cytochrome P450, subfamily IIJ, polypeptide 2 (CYP2J2) 229.10 ± 93.32 217.03 ± 90.38 NS 
Relative abundance
Fold increase
ControlAA
COX-2 14.59 ± 7.94 275.83 ± 8.14 22.44 
COX-1 4.82 ± 0.40 7.43 ± 1.31 1.55 
5-LO 16.09 ± 5.58 20.03 ± 7.03 NS 
Cytochrome P450, subfamily IIJ, polypeptide 2 (CYP2J2) 229.10 ± 93.32 217.03 ± 90.38 NS 

NOTE: Arachidonic acid (5 μg/mL) was added to down-regulated PC-3 cells. At 2 hours after the addition of arachidonic acid, RNA was collected and quantitative RT-PCR was done (n = 3).

Abbreviation: NS, not significant.

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