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Table 1.

BrdUrd and TUNEL-positive cells in papillomas and epidermis of WT and osteopontin-null mice

Male
Female
OPN+/+ (n = 7)OPN−/− (n = 5)OPN+/+ (n = 7)OPN−/− (n = 4)
BrdUrd Papilloma ++* ++ +++ +++ 
 Epidermis 3.68 ± 1.86 3.13 ± 0.67 2.95 ± 1.25 2.75 ± 2.14 
TUNEL Papilloma 9.06 ± 7.89 28.13 ± 6.71 9.81 ± 8.62 14.62 ± 5.12 
 Epidermis 0 ± 0§ 0.20 ± 0.20 0 ± 0 0.25 ± 0.25 
Male
Female
OPN+/+ (n = 7)OPN−/− (n = 5)OPN+/+ (n = 7)OPN−/− (n = 4)
BrdUrd Papilloma ++* ++ +++ +++ 
 Epidermis 3.68 ± 1.86 3.13 ± 0.67 2.95 ± 1.25 2.75 ± 2.14 
TUNEL Papilloma 9.06 ± 7.89 28.13 ± 6.71 9.81 ± 8.62 14.62 ± 5.12 
 Epidermis 0 ± 0§ 0.20 ± 0.20 0 ± 0 0.25 ± 0.25 
*

Quantitation of BrdUrd staining in papillomas. The total number of BrdUrd-stained cells from five randomly selected fields at ×40 magnification per papilloma or sample was obtained. The number obtained was then averaged by the number of mice (n) to obtain the number of BrdUrd signals per sample, which was then scored as follows: +, <20 cells; ++, between 20 and 50 cells; +++, >50 cells per sample.

Quantitation of BrdUrd staining in the epidermis surrounding papillomas. The average number of BrdUrd-positive cells from 10 fields at ×10 magnification per epidermis section or sample was obtained. The number obtained was then averaged by the number of mice (n) to obtain the number of positive signals ± SD per sample.

Quantitation of TUNEL-positive cells in papillomas. The total number of positive signals from five randomly selected fields at ×40 magnification per papilloma of similar morphology [i.e., finger, such as projections (see Fig. 4)] was obtained. The number obtained was then averaged by the number of mice (n) ± SD as indicated above.

§

Quantitation of TUNEL-positive cells in the epidermis. The average number of TUNEL-positive cells from 10 fields per epidermis section or sample was assessed. The number obtained was then averaged by the number of mice (n) ± SD as indicated above.

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