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Table 2.

NADPH-supported E1 hydroxylation activities of human CYP1A1 variants

2-OH-Estrone
4-OH-Estrone
6α-OH-Estrone
15α-OH-Estrone
VmaxKmVmax/KmVmaxKmVmax/KmVmaxKmVmax/KmVmaxKmVmax/Km
CYP1A1.1 0.4 ± 0.03 12.2 ± 1.2 0.033 0.1 ± 0.03 22.6 ± 11.7 0.004 0.2 ± 0.1 86 ± 40 0.002 1.2 ± 0.1 85 ± 12 0.014 
CYP1A1.2 2.5 ± 0.3 6.3 ± 2.0 0.397 0.5 ± 0.1 12.7 ± 1.9 0.039 0.2 ± 0.02 27.3 ± 4.6 0.008 1.0 ± 0.1 20.5 ± 5.8 0.049 
CYP1A1.4 0.3 ± 0.03 13.1 ± 3.0 0.023 0.1 ± 0.03 24.4 ± 9.8 0.004 0.1 ± 0.04 45 ± 29 0.002 1.0 ± 0.2 46 ± 21 0.022 
2-OH-Estrone
4-OH-Estrone
6α-OH-Estrone
15α-OH-Estrone
VmaxKmVmax/KmVmaxKmVmax/KmVmaxKmVmax/KmVmaxKmVmax/Km
CYP1A1.1 0.4 ± 0.03 12.2 ± 1.2 0.033 0.1 ± 0.03 22.6 ± 11.7 0.004 0.2 ± 0.1 86 ± 40 0.002 1.2 ± 0.1 85 ± 12 0.014 
CYP1A1.2 2.5 ± 0.3 6.3 ± 2.0 0.397 0.5 ± 0.1 12.7 ± 1.9 0.039 0.2 ± 0.02 27.3 ± 4.6 0.008 1.0 ± 0.1 20.5 ± 5.8 0.049 
CYP1A1.4 0.3 ± 0.03 13.1 ± 3.0 0.023 0.1 ± 0.03 24.4 ± 9.8 0.004 0.1 ± 0.04 45 ± 29 0.002 1.0 ± 0.2 46 ± 21 0.022 

NOTE: For conditions of experiments and analysis see legend of Table 1. Vmax data are given in picomoles of product per minute/picomoles of CYP1A1, Km data are in micromoles per liter, and catalytic efficiencies Vmax/Km are expressed in liter/micromoles per minute. Data represent mean ± SE as determined by nonlinear regression using software described under Materials and Methods.

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