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Table 2.

CT gene expression by RT-PCR in MSCs and bone marrow

CT gene familyTestisMelanoma
MuscleFetal MSCs
Adult MSCs
Bone marrow
Tu 379BL-Mel123412312
GAGE1, -2, -8 − − − − 
GAGE3 to -7b − − − − 
MAGE-A − − 
NY-ESO-1 − 
RAGE1, -2, -3 − − − 
SSX1 to -9 − 
CT gene familyTestisMelanoma
MuscleFetal MSCs
Adult MSCs
Bone marrow
Tu 379BL-Mel123412312
GAGE1, -2, -8 − − − − 
GAGE3 to -7b − − − − 
MAGE-A − − 
NY-ESO-1 − 
RAGE1, -2, -3 − − − 
SSX1 to -9 − 

NOTE: RT-PCR (see Material and Methods) was used to detect CT gene transcripts in testis (positive control), melanoma cell lines (Tu 379 and BL-Mel), muscle tissue (negative control), fetal MSCs (four samples), adult MSCs (three samples), and bone marrow (two samples). The table summarizes the results obtained from three independent tests. Samples were scored positive (+) if a band of the correct size was observed on any of the test occasions.

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