PCR primers designed and used to detect CT gene transcripts in tissues and cell lines
| Primer . | Specificity . | Sequence . | T (°C) . | Size (bp) . |
|---|---|---|---|---|
| SSXpan A | SSX1 to -9 | 5′-CCT CAG ATG ACT TTC GGC AGG C-′3 | 65 | 635 |
| SSXpan B | 5′-TCA CAT CTG GGG AGA GAG GAG G-′3 | |||
| SSXpan a | 5′-TCA CAT CTG GGG AGA GAG GAG G-′3 | 65 | 345 | |
| SSXpan b | 5′-GCT ATG CAC CTG ATG ACG AGG G-′3 | |||
| MAGE A | MAGE-A1, -2, -3, -5, -6, -12 | 5′-AGT CCT CAG GGA GCC TCC-′3 | 56 | 590 |
| MAGE B | 5′-CTG CCG GTA CTC CAG GT-′3 | |||
| MAGE a | 5′-CAC AAA GGC AGA AAT GCT GG-′3 | 57 | 288 | |
| MAGE b | 5′-ACT CAG CTC CTC CCA GAT TT-′3 | |||
| GAGE1, 2, 8 A | GAGE1, -2, and -8 | 5′-GAC CAA GGC GCT ATG TAC-′3 | 54 | 256 |
| GAGE1, 2, 8 B | 5′-GTC CAT CTC CTG CCC CTG-′3 | |||
| GAGE1, 2, 8 a | 5′-CGT AGA GCC TCC TGA AAT-′3 | 52 | 230 | |
| GAGE1, 2, 8 b | 5′-CCA TCA GGA CCA TCT TCA-′3 | |||
| GAGE3-7b A | GAGE3 to -7b | 5′-GAC CAA GGC GCT ATG TAC-′3 | 54 | 284 |
| GAGE3-7b B | 5′-GCG TTT TCA CCT CCT CTG-′3 | |||
| GAGE3-7b a | 5′-GCC CGA GCA GTT CAG TG-′3 | 54 | 197 | |
| GAGE3-7b b | 5′-CCA TCA GGA CCA TCT TCA-′3 | |||
| RAGE A | RAGE1, -2, and -3 | 5′-GAA CTG GAA TTG GAA GAA C-′3 | 51 | 7881 |
| RAGE B | 5′-ATA TTA GCC CAG AAC ATC C-′3 | 8422 | ||
| 8893 | ||||
| RAGE a | 5′-GGG AGA CTG AAG GGT AGC-′3 | 54 | 7011 | |
| RAGE b | 5′-AAA CGG ACG CAG GCG CAT-′3 | 7372 | ||
| 7843 | ||||
| NY-ESO-1 A | NY-ESO-1 | 5′-CCT CGC CAT GCC TTT CGC G-′3 | 62 | 381 |
| NY-ESO-1 B | 5′-CCG TCC TCC TCC AGG GA-′3 | |||
| NY-ESO-1 a | 5′-CCC CAC CGC TCC CCG TG-′3 | 68 | 274 | |
| NY-ESO-1 b | 5′-CTG GCC ACT CGT GCT GGG A-′3 |
| Primer . | Specificity . | Sequence . | T (°C) . | Size (bp) . |
|---|---|---|---|---|
| SSXpan A | SSX1 to -9 | 5′-CCT CAG ATG ACT TTC GGC AGG C-′3 | 65 | 635 |
| SSXpan B | 5′-TCA CAT CTG GGG AGA GAG GAG G-′3 | |||
| SSXpan a | 5′-TCA CAT CTG GGG AGA GAG GAG G-′3 | 65 | 345 | |
| SSXpan b | 5′-GCT ATG CAC CTG ATG ACG AGG G-′3 | |||
| MAGE A | MAGE-A1, -2, -3, -5, -6, -12 | 5′-AGT CCT CAG GGA GCC TCC-′3 | 56 | 590 |
| MAGE B | 5′-CTG CCG GTA CTC CAG GT-′3 | |||
| MAGE a | 5′-CAC AAA GGC AGA AAT GCT GG-′3 | 57 | 288 | |
| MAGE b | 5′-ACT CAG CTC CTC CCA GAT TT-′3 | |||
| GAGE1, 2, 8 A | GAGE1, -2, and -8 | 5′-GAC CAA GGC GCT ATG TAC-′3 | 54 | 256 |
| GAGE1, 2, 8 B | 5′-GTC CAT CTC CTG CCC CTG-′3 | |||
| GAGE1, 2, 8 a | 5′-CGT AGA GCC TCC TGA AAT-′3 | 52 | 230 | |
| GAGE1, 2, 8 b | 5′-CCA TCA GGA CCA TCT TCA-′3 | |||
| GAGE3-7b A | GAGE3 to -7b | 5′-GAC CAA GGC GCT ATG TAC-′3 | 54 | 284 |
| GAGE3-7b B | 5′-GCG TTT TCA CCT CCT CTG-′3 | |||
| GAGE3-7b a | 5′-GCC CGA GCA GTT CAG TG-′3 | 54 | 197 | |
| GAGE3-7b b | 5′-CCA TCA GGA CCA TCT TCA-′3 | |||
| RAGE A | RAGE1, -2, and -3 | 5′-GAA CTG GAA TTG GAA GAA C-′3 | 51 | 7881 |
| RAGE B | 5′-ATA TTA GCC CAG AAC ATC C-′3 | 8422 | ||
| 8893 | ||||
| RAGE a | 5′-GGG AGA CTG AAG GGT AGC-′3 | 54 | 7011 | |
| RAGE b | 5′-AAA CGG ACG CAG GCG CAT-′3 | 7372 | ||
| 7843 | ||||
| NY-ESO-1 A | NY-ESO-1 | 5′-CCT CGC CAT GCC TTT CGC G-′3 | 62 | 381 |
| NY-ESO-1 B | 5′-CCG TCC TCC TCC AGG GA-′3 | |||
| NY-ESO-1 a | 5′-CCC CAC CGC TCC CCG TG-′3 | 68 | 274 | |
| NY-ESO-1 b | 5′-CTG GCC ACT CGT GCT GGG A-′3 |
NOTE: PCR primers designed and used to detect CT gene transcripts in tissues and cell lines. The reaction protocol is described in Materials and Methods. A, outer forward primer; B, outer reverse primer; a, inner forward primer; b, inner reverse primer. T, PCR annealing temperature. Size, PCR product size. Superscript numbers 1, 2, and 3 refer to RAGE1, -2, and -3, respectively.