Skip to Main Content
Table 1.

PCR primers designed and used to detect CT gene transcripts in tissues and cell lines

PrimerSpecificitySequenceT (°C)Size (bp)
SSXpan A SSX1 to -9 5′-CCT CAG ATG ACT TTC GGC AGG C-′3 65 635 
SSXpan B  5′-TCA CAT CTG GGG AGA GAG GAG G-′3   
SSXpan a  5′-TCA CAT CTG GGG AGA GAG GAG G-′3 65 345 
SSXpan b  5′-GCT ATG CAC CTG ATG ACG AGG G-′3   
MAGE A MAGE-A1, -2, -3, -5, -6, -12 5′-AGT CCT CAG GGA GCC TCC-′3 56 590 
MAGE B  5′-CTG CCG GTA CTC CAG GT-′3   
MAGE a  5′-CAC AAA GGC AGA AAT GCT GG-′3 57 288 
MAGE b  5′-ACT CAG CTC CTC CCA GAT TT-′3   
GAGE1, 2, 8 A GAGE1, -2, and -8 5′-GAC CAA GGC GCT ATG TAC-′3 54 256 
GAGE1, 2, 8 B  5′-GTC CAT CTC CTG CCC CTG-′3   
GAGE1, 2, 8 a  5′-CGT AGA GCC TCC TGA AAT-′3 52 230 
GAGE1, 2, 8 b  5′-CCA TCA GGA CCA TCT TCA-′3   
GAGE3-7b A GAGE3 to -7b 5′-GAC CAA GGC GCT ATG TAC-′3 54 284 
GAGE3-7b B  5′-GCG TTT TCA CCT CCT CTG-′3   
GAGE3-7b a  5′-GCC CGA GCA GTT CAG TG-′3 54 197 
GAGE3-7b b  5′-CCA TCA GGA CCA TCT TCA-′3   
RAGE A RAGE1, -2, and -3 5′-GAA CTG GAA TTG GAA GAA C-′3 51 7881 
RAGE B  5′-ATA TTA GCC CAG AAC ATC C-′3  8422 
    8893 
RAGE a  5′-GGG AGA CTG AAG GGT AGC-′3 54 7011 
RAGE b  5′-AAA CGG ACG CAG GCG CAT-′3  7372 
    7843 
NY-ESO-1 A NY-ESO-1 5′-CCT CGC CAT GCC TTT CGC G-′3 62 381 
NY-ESO-1 B  5′-CCG TCC TCC TCC AGG GA-′3   
NY-ESO-1 a  5′-CCC CAC CGC TCC CCG TG-′3 68 274 
NY-ESO-1 b  5′-CTG GCC ACT CGT GCT GGG A-′3   
PrimerSpecificitySequenceT (°C)Size (bp)
SSXpan A SSX1 to -9 5′-CCT CAG ATG ACT TTC GGC AGG C-′3 65 635 
SSXpan B  5′-TCA CAT CTG GGG AGA GAG GAG G-′3   
SSXpan a  5′-TCA CAT CTG GGG AGA GAG GAG G-′3 65 345 
SSXpan b  5′-GCT ATG CAC CTG ATG ACG AGG G-′3   
MAGE A MAGE-A1, -2, -3, -5, -6, -12 5′-AGT CCT CAG GGA GCC TCC-′3 56 590 
MAGE B  5′-CTG CCG GTA CTC CAG GT-′3   
MAGE a  5′-CAC AAA GGC AGA AAT GCT GG-′3 57 288 
MAGE b  5′-ACT CAG CTC CTC CCA GAT TT-′3   
GAGE1, 2, 8 A GAGE1, -2, and -8 5′-GAC CAA GGC GCT ATG TAC-′3 54 256 
GAGE1, 2, 8 B  5′-GTC CAT CTC CTG CCC CTG-′3   
GAGE1, 2, 8 a  5′-CGT AGA GCC TCC TGA AAT-′3 52 230 
GAGE1, 2, 8 b  5′-CCA TCA GGA CCA TCT TCA-′3   
GAGE3-7b A GAGE3 to -7b 5′-GAC CAA GGC GCT ATG TAC-′3 54 284 
GAGE3-7b B  5′-GCG TTT TCA CCT CCT CTG-′3   
GAGE3-7b a  5′-GCC CGA GCA GTT CAG TG-′3 54 197 
GAGE3-7b b  5′-CCA TCA GGA CCA TCT TCA-′3   
RAGE A RAGE1, -2, and -3 5′-GAA CTG GAA TTG GAA GAA C-′3 51 7881 
RAGE B  5′-ATA TTA GCC CAG AAC ATC C-′3  8422 
    8893 
RAGE a  5′-GGG AGA CTG AAG GGT AGC-′3 54 7011 
RAGE b  5′-AAA CGG ACG CAG GCG CAT-′3  7372 
    7843 
NY-ESO-1 A NY-ESO-1 5′-CCT CGC CAT GCC TTT CGC G-′3 62 381 
NY-ESO-1 B  5′-CCG TCC TCC TCC AGG GA-′3   
NY-ESO-1 a  5′-CCC CAC CGC TCC CCG TG-′3 68 274 
NY-ESO-1 b  5′-CTG GCC ACT CGT GCT GGG A-′3   

NOTE: PCR primers designed and used to detect CT gene transcripts in tissues and cell lines. The reaction protocol is described in Materials and Methods. A, outer forward primer; B, outer reverse primer; a, inner forward primer; b, inner reverse primer. T, PCR annealing temperature. Size, PCR product size. Superscript numbers 1, 2, and 3 refer to RAGE1, -2, and -3, respectively.

Close Modal

or Create an Account

Close Modal
Close Modal