American Association for Cancer Research

Table 1.

Oligonucleotides used as primers in PCR and as probes in filter hybridization

SV40 DNA regions	Oligonucleotides*	Reference^† position	T°^‡	Size (bp)
Tag NH₂	PYV.for: 5′-TAGGTGCCAACCTATGGAACAGA-3′	nt 4,574-4,552	62	172
		PYV.rev: 5′-GAAAGTCTTTAGGGTCTTCTACC-3′	nt 4,403-4,425
		SV probe: 5′-ATGTTGAGAGTCAGCAGTAGCC-3′	nt 4452-4473
Regulatory	RA3: 5′-GCGTGACAGCCGGCGCAGCACC-3′	nt 358-336	64	483
		RA4: 5′-GTCCATTAGCTGCAAAGATTCCTC-3′	nt 5,118-5,142
		R probe: 5′-AATGTGTGTCAGTTAGGGTGTG-3′	nt 264-244
Human p53 gene exons 7-8	For: 5′-ATCCTGAGTAGTGGTAATCT-3′	nt 14,441-14,460	55	151
Human p53 gene exons 7-8		Rev: 5′-TACCTCGCTTAGTGCTCCCT-3′	nt 14,591-14,572

SV40 DNA regions	Oligonucleotides*	Reference^† position	T°^‡	Size (bp)
Tag NH₂	PYV.for: 5′-TAGGTGCCAACCTATGGAACAGA-3′	nt 4,574-4,552	62	172
		PYV.rev: 5′-GAAAGTCTTTAGGGTCTTCTACC-3′	nt 4,403-4,425
		SV probe: 5′-ATGTTGAGAGTCAGCAGTAGCC-3′	nt 4452-4473
Regulatory	RA3: 5′-GCGTGACAGCCGGCGCAGCACC-3′	nt 358-336	64	483
		RA4: 5′-GTCCATTAGCTGCAAAGATTCCTC-3′	nt 5,118-5,142
		R probe: 5′-AATGTGTGTCAGTTAGGGTGTG-3′	nt 264-244
Human p53 gene exons 7-8	For: 5′-ATCCTGAGTAGTGGTAATCT-3′	nt 14,441-14,460	55	151
Human p53 gene exons 7-8		Rev: 5′-TACCTCGCTTAGTGCTCCCT-3′	nt 14,591-14,572

Oligonucleotides used as primers in PCR and as probes in filter hybridizations.

†

Reference nucleotide positions in SV40 strain 776 and human p53 gene as a control of DNA suitability (European Molecular Biology Laboratory/Genbank accession no. X54156).

‡

PCR annealing temperature (°C).

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