Table 1.

Gene-specific PCR primer sets for the genes in low abundance

No.Clone IDDirectionSequence (5′→3′)Size of PCR Product (bp)Annealing Temperature (°C)/Cycles
1 HA50C1 F 5′-GGGTGGTATTGATGGAGT-3′ 503 42/35 
  R 5′-CTTGGGGGCATACAACTC-3′   
2 HA52C2 F 5′-CCAAGGGGAACTTCCTCG-3′ 307 60/35 
  R 5′-TGGTCTGAGATGCCTGCC-3′   
3 HA62A2 F 5′-CAGGCGCCTACTTAACCT-3′ 424 47/35 
  R 5′-ACATGGTACCACTGCCGT-3′   
4 HA73C1 F 5′-AATGGAAAGTTGGGCAGG-3′ 120 47/35 
  R 5′-GGGTTTCACTGTGTTAGCC-3′   
5 HC2C1 F 5′-ACACACTGCCAGTCTTCCC-3′ 342 50/30 
  R 5′-TGGCCCTTTCAGTACAGGC-3′   
6 HC15C2 F 5′-AGGCTGAAGTGCAGCGAT-3′ 262 50/30 
  R 5′-AACTGATTTCGGCCGGGT-3′   
7 HC26C1 F 5′-GGCTGGAACTTCAGGGTCAG-3′ 473 60/35 
  R 5′-GCTCCAGTGACTGTTGAGCC-3′   
8 HC65A1 F 5′-CCACTCTCCAGATGCTAAGC-3′ 402 55/35 
  R 5′-GCGTTGCAAAGGACAGATCC-3′   
9 HC71A1 F 5′-CTCCTGAAGGACAAAGTG-3′ 240 42/35 
  R 5′-CTAGTAACGGAGGAACAG-3′   
10 HG33C1 F 5′-TCCATGCTCATGGGTAGGA-3′ 162 50/35 
  R 5′-GTCTTGGCAATATGGGCTC-3′   
No.Clone IDDirectionSequence (5′→3′)Size of PCR Product (bp)Annealing Temperature (°C)/Cycles
1 HA50C1 F 5′-GGGTGGTATTGATGGAGT-3′ 503 42/35 
  R 5′-CTTGGGGGCATACAACTC-3′   
2 HA52C2 F 5′-CCAAGGGGAACTTCCTCG-3′ 307 60/35 
  R 5′-TGGTCTGAGATGCCTGCC-3′   
3 HA62A2 F 5′-CAGGCGCCTACTTAACCT-3′ 424 47/35 
  R 5′-ACATGGTACCACTGCCGT-3′   
4 HA73C1 F 5′-AATGGAAAGTTGGGCAGG-3′ 120 47/35 
  R 5′-GGGTTTCACTGTGTTAGCC-3′   
5 HC2C1 F 5′-ACACACTGCCAGTCTTCCC-3′ 342 50/30 
  R 5′-TGGCCCTTTCAGTACAGGC-3′   
6 HC15C2 F 5′-AGGCTGAAGTGCAGCGAT-3′ 262 50/30 
  R 5′-AACTGATTTCGGCCGGGT-3′   
7 HC26C1 F 5′-GGCTGGAACTTCAGGGTCAG-3′ 473 60/35 
  R 5′-GCTCCAGTGACTGTTGAGCC-3′   
8 HC65A1 F 5′-CCACTCTCCAGATGCTAAGC-3′ 402 55/35 
  R 5′-GCGTTGCAAAGGACAGATCC-3′   
9 HC71A1 F 5′-CTCCTGAAGGACAAAGTG-3′ 240 42/35 
  R 5′-CTAGTAACGGAGGAACAG-3′   
10 HG33C1 F 5′-TCCATGCTCATGGGTAGGA-3′ 162 50/35 
  R 5′-GTCTTGGCAATATGGGCTC-3′   
View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.
Accept