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Table 1.

Characterization of the ras-dependent paracrine mediator of TSP-1 down-regulation

Medium	Treatment	Mean MTSP-1 promoter activity (MDF528 cells), %	P (t test)
Control medium (serum free)	None	100 ± 12*	—
528ras1 conditioned medium fraction <3 kDa	None	30 ± 13^†	<0.000001*
528ras1 conditioned medium fraction <3 kDa	37°C for 2 h	42 ± 20	<0.0001*
528ras1 conditioned medium fraction <3 kDa	37°C for 2 h	42 ± 20				<0.0005^‡
528ras1 conditioned medium fraction <3 kDa	85°C for 2 h	104 ± 9	<0.0005^‡
528ras1 conditioned medium fraction <3 kDa	Trypsin for 2 h	14 ± 14	<0.000001*

Medium	Treatment	Mean MTSP-1 promoter activity (MDF528 cells), %	P (t test)
Control medium (serum free)	None	100 ± 12*	—
528ras1 conditioned medium fraction <3 kDa	None	30 ± 13^†	<0.000001*
528ras1 conditioned medium fraction <3 kDa	37°C for 2 h	42 ± 20	<0.0001*
528ras1 conditioned medium fraction <3 kDa	37°C for 2 h	42 ± 20				<0.0005^‡
528ras1 conditioned medium fraction <3 kDa	85°C for 2 h	104 ± 9	<0.0005^‡
528ras1 conditioned medium fraction <3 kDa	Trypsin for 2 h	14 ± 14	<0.000001*

Compared with control medium.

Highly significant.

Compared with 528ras1 conditioned medium fraction <3 kDa.