American Association for Cancer Research

Table 2.

Estimated values of blood half-times for the T84.66 cFv-Fcγ1 fragments

Antibody fragment	t_1/2α (h)	A_α* (%ID/g)	t_1/2β (h)	A_β (%ID/g)	Area under the curve^†	Mean residence time (h)^‡
Wild-type	4.22	23.3	289	22.4	9,480	411
H435R	1.87	19.5	83.4	26.6	3,250	118
H435Q	1.53	20.9	52.6	28.0	2,170	74
I253A	2.31	28.9	51.7	18.8	1,497	70
H310A	1.66	32.6	27.2	20.2	873	36
H310A/H435Q	0.86	25.2	8.00	32.3	402	11

Antibody fragment	t_1/2α (h)	A_α* (%ID/g)	t_1/2β (h)	A_β (%ID/g)	Area under the curve^†	Mean residence time (h)^‡
Wild-type	4.22	23.3	289	22.4	9,480	411
H435R	1.87	19.5	83.4	26.6	3,250	118
H435Q	1.53	20.9	52.6	28.0	2,170	74
I253A	2.31	28.9	51.7	18.8	1,497	70
H310A	1.66	32.6	27.2	20.2	873	36
H310A/H435Q	0.86	25.2	8.00	32.3	402	11

Amplitudes of the two components are given by A_α and A_β, where the sum of A_α and A_β is the total %ID/g.

Area under the curve is a time integral of the blood uptake (%ID/g × h).

Mean residence time used to give a single variable for blood clearance.