Microvessel density, area of the vessels, and tumor infiltration after isolated perfusion with sham, IL-2, melphalan, or IL-2 plus melphalan
| . | Sham . | IL-2 . | Melphalan . | IL-2 + melphalan . |
|---|---|---|---|---|
| No. vessels* | 33 ± 9 | 64 ± 24 | 22 ± 2 | 29 ± 5 |
| Area of endothelium† | 6.8 ± 0.6 | 9.2 ± 1.3 | 5.9 ± 0.9 | 6.4 ± 0.8 |
| Area endothelium/vessel‡ | 0.30 ± 0.09 | 0.16 ± 0.04 | 0.28 ± 0.03 | 0.24 ± 0.02 |
| CD4§ | 0.4 ± 0.2 | 2.4 ± 0.2 | 0.2 ± 0.2 | 0.1 ± 0.1 |
| CD8§ | 51 ± 7 | 36 ± 10 | 67 ± 47 | 37 ± 10 |
| Granulocytes§ | 60 ± 4 | 79 ± 4∥ | 48 ± 3∥ | 63 ± 4¶ |
| Macrophages§ | 210 ± 7 | 222 ± 52 | 201 ± 20 | 189 ± 20 |
| . | Sham . | IL-2 . | Melphalan . | IL-2 + melphalan . |
|---|---|---|---|---|
| No. vessels* | 33 ± 9 | 64 ± 24 | 22 ± 2 | 29 ± 5 |
| Area of endothelium† | 6.8 ± 0.6 | 9.2 ± 1.3 | 5.9 ± 0.9 | 6.4 ± 0.8 |
| Area endothelium/vessel‡ | 0.30 ± 0.09 | 0.16 ± 0.04 | 0.28 ± 0.03 | 0.24 ± 0.02 |
| CD4§ | 0.4 ± 0.2 | 2.4 ± 0.2 | 0.2 ± 0.2 | 0.1 ± 0.1 |
| CD8§ | 51 ± 7 | 36 ± 10 | 67 ± 47 | 37 ± 10 |
| Granulocytes§ | 60 ± 4 | 79 ± 4∥ | 48 ± 3∥ | 63 ± 4¶ |
| Macrophages§ | 210 ± 7 | 222 ± 52 | 201 ± 20 | 189 ± 20 |
NOTE: Directly after ILP, the tumors were excised and frozen sections were stained for granulocytes, macrophages, and CD31-, CD4-, and CD8-positive cells. At least two animals per group and six fields of interest per tumor were evaluated. Average ± SE is shown.
No. vessels per field of interest.
% Total vessel area per field of interest.
Area per vessel.
Amount of positive cells per field of interest.
P = 0.05 compared with sham treatment.
P = 0.05 compared with melphalan treatment.