RT-PCR primers for the immune-related genes and for β-actin, which was used as a housekeeping gene
| Gene . | Primers . | Annealing temperature (°C) . | Product size (bp) . |
|---|---|---|---|
| β-actin | f, 5′-ATGGATGACGATATCGCTG-3′ | 60 | 569 |
| r, 5′-ATGAGGTAGTCTGTCAGGT-3′ | |||
| IL-6 | f, 5′-GACTTCACAGAGGATACC-3′ | 55 | 294 |
| r, 5′-TAAGTTGTTCTTCACAAACTCC-3′ | |||
| GRO/CINC-A | f, 5′-GAAGATAGATTGCACCGATG-3′ | 57 | 367 |
| r, 5′-CATAGCCTCTCACACATTTC-3′ | |||
| IL-10 | f, 5′-TGACAATAACTGCACCCACTT-3′ | 60 | 402 |
| r, 5′-TCATTCATGGCCTTGTAGACA-3′ | |||
| IL-12 | f, 5′-TCATCAGGGACATCATCAAACC-3′ | 65 | 210 |
| r, 5′-CGAGGAACGCACCTTTCTG-3′ | |||
| TNF-α | f, 5′-TACTGAACTTCGGGGTGATCGGTCC-3′ | 60 | 295 |
| r, 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′ | |||
| IFN-γ | f, 5′-GCCTCCTCTTGGATATCTGG-3′ | 60 | 239 |
| r, 5′-GTGCTGGATCTGTGGGTTG-3′ | |||
| MCP-1 | f, 5′-ATGCAGGTCTCTCTGTCACG-3′ | 57 | 446 |
| r, 5′-CTAGTTCTCTGTCATACT-3′ | |||
| MIP-2 | f, 5′-GGCACAATCGGTACGATCCAG-3′ | 55 | 287 |
| r, 5′-ACCCTGCCAAGGGTTGACTTC-3′ | |||
| TGF-β1 | f, 5′-TGGAAGTGGATCCACGAGCCCAAG-3′ | 55 | 240 |
| r, 5′-GCAGGAGCGCACGATCATGTTGGAC-3′ |
| Gene . | Primers . | Annealing temperature (°C) . | Product size (bp) . |
|---|---|---|---|
| β-actin | f, 5′-ATGGATGACGATATCGCTG-3′ | 60 | 569 |
| r, 5′-ATGAGGTAGTCTGTCAGGT-3′ | |||
| IL-6 | f, 5′-GACTTCACAGAGGATACC-3′ | 55 | 294 |
| r, 5′-TAAGTTGTTCTTCACAAACTCC-3′ | |||
| GRO/CINC-A | f, 5′-GAAGATAGATTGCACCGATG-3′ | 57 | 367 |
| r, 5′-CATAGCCTCTCACACATTTC-3′ | |||
| IL-10 | f, 5′-TGACAATAACTGCACCCACTT-3′ | 60 | 402 |
| r, 5′-TCATTCATGGCCTTGTAGACA-3′ | |||
| IL-12 | f, 5′-TCATCAGGGACATCATCAAACC-3′ | 65 | 210 |
| r, 5′-CGAGGAACGCACCTTTCTG-3′ | |||
| TNF-α | f, 5′-TACTGAACTTCGGGGTGATCGGTCC-3′ | 60 | 295 |
| r, 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′ | |||
| IFN-γ | f, 5′-GCCTCCTCTTGGATATCTGG-3′ | 60 | 239 |
| r, 5′-GTGCTGGATCTGTGGGTTG-3′ | |||
| MCP-1 | f, 5′-ATGCAGGTCTCTCTGTCACG-3′ | 57 | 446 |
| r, 5′-CTAGTTCTCTGTCATACT-3′ | |||
| MIP-2 | f, 5′-GGCACAATCGGTACGATCCAG-3′ | 55 | 287 |
| r, 5′-ACCCTGCCAAGGGTTGACTTC-3′ | |||
| TGF-β1 | f, 5′-TGGAAGTGGATCCACGAGCCCAAG-3′ | 55 | 240 |
| r, 5′-GCAGGAGCGCACGATCATGTTGGAC-3′ |
Abbreviations: f, forward primer; r, reverse primer.