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Table 1.

RT-PCR primers for the immune-related genes and for β-actin, which was used as a housekeeping gene

GenePrimersAnnealing temperature (°C)Product size (bp)
β-actin f, 5′-ATGGATGACGATATCGCTG-3′ 60 569 
 r, 5′-ATGAGGTAGTCTGTCAGGT-3′   
IL-6 f, 5′-GACTTCACAGAGGATACC-3′ 55 294 
 r, 5′-TAAGTTGTTCTTCACAAACTCC-3′   
GRO/CINC-A f, 5′-GAAGATAGATTGCACCGATG-3′ 57 367 
 r, 5′-CATAGCCTCTCACACATTTC-3′   
IL-10 f, 5′-TGACAATAACTGCACCCACTT-3′ 60 402 
 r, 5′-TCATTCATGGCCTTGTAGACA-3′   
IL-12 f, 5′-TCATCAGGGACATCATCAAACC-3′ 65 210 
 r, 5′-CGAGGAACGCACCTTTCTG-3′   
TNF-α f, 5′-TACTGAACTTCGGGGTGATCGGTCC-3′ 60 295 
 r, 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′   
IFN-γ f, 5′-GCCTCCTCTTGGATATCTGG-3′ 60 239 
 r, 5′-GTGCTGGATCTGTGGGTTG-3′   
MCP-1 f, 5′-ATGCAGGTCTCTCTGTCACG-3′ 57 446 
 r, 5′-CTAGTTCTCTGTCATACT-3′   
MIP-2 f, 5′-GGCACAATCGGTACGATCCAG-3′ 55 287 
 r, 5′-ACCCTGCCAAGGGTTGACTTC-3′   
TGF-β1 f, 5′-TGGAAGTGGATCCACGAGCCCAAG-3′ 55 240 
 r, 5′-GCAGGAGCGCACGATCATGTTGGAC-3′   
GenePrimersAnnealing temperature (°C)Product size (bp)
β-actin f, 5′-ATGGATGACGATATCGCTG-3′ 60 569 
 r, 5′-ATGAGGTAGTCTGTCAGGT-3′   
IL-6 f, 5′-GACTTCACAGAGGATACC-3′ 55 294 
 r, 5′-TAAGTTGTTCTTCACAAACTCC-3′   
GRO/CINC-A f, 5′-GAAGATAGATTGCACCGATG-3′ 57 367 
 r, 5′-CATAGCCTCTCACACATTTC-3′   
IL-10 f, 5′-TGACAATAACTGCACCCACTT-3′ 60 402 
 r, 5′-TCATTCATGGCCTTGTAGACA-3′   
IL-12 f, 5′-TCATCAGGGACATCATCAAACC-3′ 65 210 
 r, 5′-CGAGGAACGCACCTTTCTG-3′   
TNF-α f, 5′-TACTGAACTTCGGGGTGATCGGTCC-3′ 60 295 
 r, 5′-CAGCCTTGTCCCTTGAAGAGAACC-3′   
IFN-γ f, 5′-GCCTCCTCTTGGATATCTGG-3′ 60 239 
 r, 5′-GTGCTGGATCTGTGGGTTG-3′   
MCP-1 f, 5′-ATGCAGGTCTCTCTGTCACG-3′ 57 446 
 r, 5′-CTAGTTCTCTGTCATACT-3′   
MIP-2 f, 5′-GGCACAATCGGTACGATCCAG-3′ 55 287 
 r, 5′-ACCCTGCCAAGGGTTGACTTC-3′   
TGF-β1 f, 5′-TGGAAGTGGATCCACGAGCCCAAG-3′ 55 240 
 r, 5′-GCAGGAGCGCACGATCATGTTGGAC-3′   

Abbreviations: f, forward primer; r, reverse primer.

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