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Table 1

Summary of methylation-specific PCR (MSP) analysis of normal DNA from individuals without breast cancer and distal-to-tumor tissue from women with ductal carcinoma in situ (DCIS)

SamplesUpstreamExon 1 Nested MSP
Standard MSPNested MSP
Purified Epithelium    
 N1a 
 N2  
 N3  
 N4 
Whole breast    
 N5 
 N6 
 N7 
Peripheral to DCIS    
 DCIS N8 
 DCIS N9 
 DCIS N10 
 DCIS N11 
 DCIS N12 
 DCIS N13 
 DCIS N14 
 DCIS N15 
 DCIS N16  
SamplesUpstreamExon 1 Nested MSP
Standard MSPNested MSP
Purified Epithelium    
 N1a 
 N2  
 N3  
 N4 
Whole breast    
 N5 
 N6 
 N7 
Peripheral to DCIS    
 DCIS N8 
 DCIS N9 
 DCIS N10 
 DCIS N11 
 DCIS N12 
 DCIS N13 
 DCIS N14 
 DCIS N15 
 DCIS N16  
a

N numbers, preparations of breast cells taken from different women at time of a mammoplasty procedure; U, unmethylated; M, methylated.

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