Summary of methylation-specific PCR (MSP) analysis of normal DNA from individuals without breast cancer and distal-to-tumor tissue from women with ductal carcinoma in situ (DCIS)
| Samples . | Upstream . | . | Exon 1 Nested MSP . | |
|---|---|---|---|---|
| . | Standard MSP . | Nested MSP . | . | |
| Purified Epithelium | ||||
| N1a | U | U | U | |
| N2 | U | U | ||
| N3 | U | U | ||
| N4 | U | M | U | |
| Whole breast | ||||
| N5 | U | M | U | |
| N6 | U | U | U | |
| N7 | U | M | U | |
| Peripheral to DCIS | ||||
| DCIS N8 | M | M | U | |
| DCIS N9 | U | U | U | |
| DCIS N10 | U | U | U | |
| DCIS N11 | U | U | U | |
| DCIS N12 | U | U | U | |
| DCIS N13 | U | M | U | |
| DCIS N14 | U | M | U | |
| DCIS N15 | U | U | U | |
| DCIS N16 | U | U | ||
| Samples . | Upstream . | . | Exon 1 Nested MSP . | |
|---|---|---|---|---|
| . | Standard MSP . | Nested MSP . | . | |
| Purified Epithelium | ||||
| N1a | U | U | U | |
| N2 | U | U | ||
| N3 | U | U | ||
| N4 | U | M | U | |
| Whole breast | ||||
| N5 | U | M | U | |
| N6 | U | U | U | |
| N7 | U | M | U | |
| Peripheral to DCIS | ||||
| DCIS N8 | M | M | U | |
| DCIS N9 | U | U | U | |
| DCIS N10 | U | U | U | |
| DCIS N11 | U | U | U | |
| DCIS N12 | U | U | U | |
| DCIS N13 | U | M | U | |
| DCIS N14 | U | M | U | |
| DCIS N15 | U | U | U | |
| DCIS N16 | U | U | ||
N numbers, preparations of breast cells taken from different women at time of a mammoplasty procedure; U, unmethylated; M, methylated.