Inhibitory effect of endostatin and endostatin heparin-binding mutants on chicken CAM angiogenesis induced by FGF-2 or VEGF-A
| Stimulator . | Inhibitor . | Angiogenesis score (mean) . | No. of embryos . |
|---|---|---|---|
| Buffer | None | 1.1 | 8 |
| FGF-2, 0.2 μg | None | 3.2 | 8 |
| ES, 0.3 μg | 3.0 | 5 | |
| ES, 3 μg | 1.1 | 8 | |
| VEGF-A, 0.2 μg | None | 3.3 | 8 |
| ES, 3 μg | 3.2 | 5 | |
| ES, 15 μg | 2.0 | 7 | |
| FGF-2, 0.2 μg | R158/184/270A, 3 μg | 2.8 | 7 |
| R158/184/270A, 15 μg | 3.0 | 5 | |
| R193/194A, 3 μg | 2.9 | 7 | |
| VEGF-A, 0.2 μg | R158/184/270A, 15 μg | 1.6 | 7 |
| R193/194A, 15 μg | 3.0 | 5 |
| Stimulator . | Inhibitor . | Angiogenesis score (mean) . | No. of embryos . |
|---|---|---|---|
| Buffer | None | 1.1 | 8 |
| FGF-2, 0.2 μg | None | 3.2 | 8 |
| ES, 0.3 μg | 3.0 | 5 | |
| ES, 3 μg | 1.1 | 8 | |
| VEGF-A, 0.2 μg | None | 3.3 | 8 |
| ES, 3 μg | 3.2 | 5 | |
| ES, 15 μg | 2.0 | 7 | |
| FGF-2, 0.2 μg | R158/184/270A, 3 μg | 2.8 | 7 |
| R158/184/270A, 15 μg | 3.0 | 5 | |
| R193/194A, 3 μg | 2.9 | 7 | |
| VEGF-A, 0.2 μg | R158/184/270A, 15 μg | 1.6 | 7 |
| R193/194A, 15 μg | 3.0 | 5 |