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Table 4

Inhibition of PMA-induced macrophage differentiation of HL-60 cells by SSAT antisense oligonucleotides

Attached and spread cells (%)Phagocytic cells (%)
HL-60 cells treated with   
 None <5 <5 
 PMA 79 87 
 2 μmol/L spermine <5 <5 
 PMA + 2 μmol/L spermine 81 78 
 PMA + 2 μmol/L spermine + antisense oligonucleotides 21 
 PMA + 2 μmol/L spermine + control oligonucleotides 72 86 
Attached and spread cells (%)Phagocytic cells (%)
HL-60 cells treated with   
 None <5 <5 
 PMA 79 87 
 2 μmol/L spermine <5 <5 
 PMA + 2 μmol/L spermine 81 78 
 PMA + 2 μmol/L spermine + antisense oligonucleotides 21 
 PMA + 2 μmol/L spermine + control oligonucleotides 72 86 

NOTE. The data from a representative experiment out of three are shown. The concentration of PMA and oligonucleotides were 3 nmol/L and 100 μmol/L, respectively. Differentiation markers were determined at 21 hours after PMA treatment.

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