Inhibition of PMA-induced macrophage differentiation of HL-60 cells by SSAT antisense oligonucleotides
| . | Attached and spread cells (%) . | Phagocytic cells (%) . |
|---|---|---|
| HL-60 cells treated with | ||
| None | <5 | <5 |
| PMA | 79 | 87 |
| 2 μmol/L spermine | <5 | <5 |
| PMA + 2 μmol/L spermine | 81 | 78 |
| PMA + 2 μmol/L spermine + antisense oligonucleotides | 9 | 21 |
| PMA + 2 μmol/L spermine + control oligonucleotides | 72 | 86 |
| . | Attached and spread cells (%) . | Phagocytic cells (%) . |
|---|---|---|
| HL-60 cells treated with | ||
| None | <5 | <5 |
| PMA | 79 | 87 |
| 2 μmol/L spermine | <5 | <5 |
| PMA + 2 μmol/L spermine | 81 | 78 |
| PMA + 2 μmol/L spermine + antisense oligonucleotides | 9 | 21 |
| PMA + 2 μmol/L spermine + control oligonucleotides | 72 | 86 |
NOTE. The data from a representative experiment out of three are shown. The concentration of PMA and oligonucleotides were 3 nmol/L and 100 μmol/L, respectively. Differentiation markers were determined at 21 hours after PMA treatment.