Inhibition of PMA-induced macrophage differentiation of HL-60 and U-937 cells by exogenous spermine in presence or absence of AG
| . | Cells concentration (×103 cells/mL) . | . | Attached and spread cells (%) . | . | Phagocytic cells (%) . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | Control . | AG . | Control . | AG . | Control . | AG . | |||
| HL-60 cells treated with | |||||||||
| None | 38 | 31 | <5 | <5 | <3 | <3 | |||
| PMA | 24 | 23 | 77 | 73 | 74 | 68 | |||
| 4 μmol/L spermine | 18 | 31 | <5 | <5 | <3 | <3 | |||
| 4 μmol/L spermine + PMA | 14 | 22 | 28 | 17 | 22 | 15 | |||
| U-937 cells treated with | |||||||||
| None | 42 | 34 | <5 | <5 | <5 | <5 | |||
| PMA | 22 | 22 | 86 | 84 | 80 | 79 | |||
| 4 μmol/L spermine | 20 | 35 | <5 | <5 | <5 | <5 | |||
| 4 μmol/L spermine + PMA | 15 | 23 | 25 | 21 | 18 | 17 | |||
| . | Cells concentration (×103 cells/mL) . | . | Attached and spread cells (%) . | . | Phagocytic cells (%) . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | Control . | AG . | Control . | AG . | Control . | AG . | |||
| HL-60 cells treated with | |||||||||
| None | 38 | 31 | <5 | <5 | <3 | <3 | |||
| PMA | 24 | 23 | 77 | 73 | 74 | 68 | |||
| 4 μmol/L spermine | 18 | 31 | <5 | <5 | <3 | <3 | |||
| 4 μmol/L spermine + PMA | 14 | 22 | 28 | 17 | 22 | 15 | |||
| U-937 cells treated with | |||||||||
| None | 42 | 34 | <5 | <5 | <5 | <5 | |||
| PMA | 22 | 22 | 86 | 84 | 80 | 79 | |||
| 4 μmol/L spermine | 20 | 35 | <5 | <5 | <5 | <5 | |||
| 4 μmol/L spermine + PMA | 15 | 23 | 25 | 21 | 18 | 17 | |||
NOTE. The data from a representative experiment out of two are shown. Cell concentration was determined at 36 hours after treatment with or without 3 nmol/L PMA while cell attachment and spreading and phagocytosis at 21 hours after this treatment. The final concentration of AG was 1 mmol/L.