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Table 2

Inhibition of PMA-induced macrophage differentiation of HL-60 and U-937 cells by exogenous spermine in presence or absence of AG

Cells concentration (×103 cells/mL)Attached and spread cells (%)Phagocytic cells (%)
ControlAGControlAGControlAG
HL-60 cells treated with       
 None 38 31 <5 <5 <3 <3 
 PMA 24 23 77 73 74 68 
 4 μmol/L spermine 18 31 <5 <5 <3 <3 
 4 μmol/L spermine + PMA 14 22 28 17 22 15 
U-937 cells treated with       
 None 42 34 <5 <5 <5 <5 
 PMA 22 22 86 84 80 79 
 4 μmol/L spermine 20 35 <5 <5 <5 <5 
 4 μmol/L spermine + PMA 15 23 25 21 18 17 
Cells concentration (×103 cells/mL)Attached and spread cells (%)Phagocytic cells (%)
ControlAGControlAGControlAG
HL-60 cells treated with       
 None 38 31 <5 <5 <3 <3 
 PMA 24 23 77 73 74 68 
 4 μmol/L spermine 18 31 <5 <5 <3 <3 
 4 μmol/L spermine + PMA 14 22 28 17 22 15 
U-937 cells treated with       
 None 42 34 <5 <5 <5 <5 
 PMA 22 22 86 84 80 79 
 4 μmol/L spermine 20 35 <5 <5 <5 <5 
 4 μmol/L spermine + PMA 15 23 25 21 18 17 

NOTE. The data from a representative experiment out of two are shown. Cell concentration was determined at 36 hours after treatment with or without 3 nmol/L PMA while cell attachment and spreading and phagocytosis at 21 hours after this treatment. The final concentration of AG was 1 mmol/L.

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