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Table 1

Inhibition of PMA-induced macrophage differentiation of HL-60 and U-937 cells by exogenous spermine

Attached and spread cells (%)Phagocytic cells (%)
HL-60 cells treated with   
 None <5 <3 
 PMA 83 78 
 4 μmol/L spermine <5 <3 
 4 μmol/L spermine + PMA 20 22 
 10 μmol/L spermidine <5 <3 
 10 μmol/L spermidine + PMA 73 76 
 10 μmol/L putrescine <5 <3 
 10 μmol/L putrescine + PMA 70 76 
U937 cells treated with   
 None 
 PMA 88 81 
 4 μmol/L spermine <5 <5 
 4 μmol/L spermine + PMA 27 16 
 10 μmol/L spermidine <5 <5 
 10 μmol/L spermidine + PMA 83 77 
 10 μmol/L putrescine <5 <5 
 10 μmol/L putrescine + PMA 84 82 
Attached and spread cells (%)Phagocytic cells (%)
HL-60 cells treated with   
 None <5 <3 
 PMA 83 78 
 4 μmol/L spermine <5 <3 
 4 μmol/L spermine + PMA 20 22 
 10 μmol/L spermidine <5 <3 
 10 μmol/L spermidine + PMA 73 76 
 10 μmol/L putrescine <5 <3 
 10 μmol/L putrescine + PMA 70 76 
U937 cells treated with   
 None 
 PMA 88 81 
 4 μmol/L spermine <5 <5 
 4 μmol/L spermine + PMA 27 16 
 10 μmol/L spermidine <5 <5 
 10 μmol/L spermidine + PMA 83 77 
 10 μmol/L putrescine <5 <5 
 10 μmol/L putrescine + PMA 84 82 

NOTE. The data from a representative experiment out of three are shown. The differentiation markers were determined at 21 hours after treatment with 3 nmol/L PMA.

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