American Association for Cancer Research

Table 1

Inhibition of PMA-induced macrophage differentiation of HL-60 and U-937 cells by exogenous spermine

	Attached and spread cells (%)	Phagocytic cells (%)
HL-60 cells treated with
None	<5	<3
PMA	83	78
4 μmol/L spermine	<5	<3
4 μmol/L spermine + PMA	20	22
10 μmol/L spermidine	<5	<3
10 μmol/L spermidine + PMA	73	76
10 μmol/L putrescine	<5	<3
10 μmol/L putrescine + PMA	70	76
U937 cells treated with
None	5	6
PMA	88	81
4 μmol/L spermine	<5	<5
4 μmol/L spermine + PMA	27	16
10 μmol/L spermidine	<5	<5
10 μmol/L spermidine + PMA	83	77
10 μmol/L putrescine	<5	<5
10 μmol/L putrescine + PMA	84	82

	Attached and spread cells (%)	Phagocytic cells (%)
HL-60 cells treated with
None	<5	<3
PMA	83	78
4 μmol/L spermine	<5	<3
4 μmol/L spermine + PMA	20	22
10 μmol/L spermidine	<5	<3
10 μmol/L spermidine + PMA	73	76
10 μmol/L putrescine	<5	<3
10 μmol/L putrescine + PMA	70	76
U937 cells treated with
None	5	6
PMA	88	81
4 μmol/L spermine	<5	<5
4 μmol/L spermine + PMA	27	16
10 μmol/L spermidine	<5	<5
10 μmol/L spermidine + PMA	83	77
10 μmol/L putrescine	<5	<5
10 μmol/L putrescine + PMA	84	82

NOTE. The data from a representative experiment out of three are shown. The differentiation markers were determined at 21 hours after treatment with 3 nmol/L PMA.

View Large

This Feature Is Available To Subscribers Only