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Table 3

DCB-3500 and DCB-3503 induce S-phase accumulation in KB cells but not in HepG2 cells

KB and HepG2 cells were treated with two concentrations of DCB-3500 and DCB-3503 for 24 h. At the end of treatment, cells were washed, resuspended in PBS, and stained with PI containing RNase A for flow cytometric analysis. Data were analyzed using Modfit software. Results are means ± SD of three independent experiments. Student’s t test was used to examine the significance of the data. For S phase in KB cells, 0.03 μm DCB-3500 versus control, P < 0.05; 0.3 μm DCB-3500 versus 0.03 μm DCB-3500, P < 0.05; 0.3 μm DCB-3503 versus control, P < 0.05; but there was no significant difference between 1 μm DCB-3503 and 0.3 μm DCB-3503.

KBHepG2
G0–G1 (%)S (%)G2–M (%)G0–G1 (%)S (%)G2–M (%)
Control 68 ± 10 12 ± 5 19 ± 5 46 ± 4 25 ± 9 29 ± 5 
Hydroxyurea 2 mm 70 ± 7 26 ± 1 9 ± 5 49 ± 10 33 ± 17 19 ± 10 
Nocodazole 0.1 μg/ml 6 ± 3 14 ± 3 81 ± 10 4 ± 3 13 ± 8 83 ± 10 
DCB-3500 0.03 μm 62 ± 1 21 ± 4 16 ± 4 45 ± 5 24 ± 8 31 ± 6 
DCB-3500 0.3 μm 55 ± 9 32 ± 6 12 ± 6 55 ± 5 17 ± 6 25 ± 5 
DCB-3503 0.3 μm 59 ± 7 24 ± 7 18 ± 3 52 ± 4 22 ± 4 25 ± 6 
DCB-3503 1 μm 63 ± 1 24 ± 1 13 ± 1 50 ± 8 21 ± 8 29 ± 2 
KBHepG2
G0–G1 (%)S (%)G2–M (%)G0–G1 (%)S (%)G2–M (%)
Control 68 ± 10 12 ± 5 19 ± 5 46 ± 4 25 ± 9 29 ± 5 
Hydroxyurea 2 mm 70 ± 7 26 ± 1 9 ± 5 49 ± 10 33 ± 17 19 ± 10 
Nocodazole 0.1 μg/ml 6 ± 3 14 ± 3 81 ± 10 4 ± 3 13 ± 8 83 ± 10 
DCB-3500 0.03 μm 62 ± 1 21 ± 4 16 ± 4 45 ± 5 24 ± 8 31 ± 6 
DCB-3500 0.3 μm 55 ± 9 32 ± 6 12 ± 6 55 ± 5 17 ± 6 25 ± 5 
DCB-3503 0.3 μm 59 ± 7 24 ± 7 18 ± 3 52 ± 4 22 ± 4 25 ± 6 
DCB-3503 1 μm 63 ± 1 24 ± 1 13 ± 1 50 ± 8 21 ± 8 29 ± 2 
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