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Table 1

Expression of γH2AXa in Hoechst 33342 stained cells

Drug treatmentRelative amount of γH2AX in 20% dimmest Hoechst stained pixelsbRelative amount of γH2AX in 20% brightest Hoechst stained pixelsRatio of γH2AX in perfused versus nonperfused regions
Etoposide 0.23 (0.003)c 0.62 (0.05) 2.6 
Tirapazamine 0.35 (0.005) 0.14 (0.006) 0.4 
Drug treatmentRelative amount of γH2AX in 20% dimmest Hoechst stained pixelsbRelative amount of γH2AX in 20% brightest Hoechst stained pixelsRatio of γH2AX in perfused versus nonperfused regions
Etoposide 0.23 (0.003)c 0.62 (0.05) 2.6 
Tirapazamine 0.35 (0.005) 0.14 (0.006) 0.4 
a

γH2AX, serine 139 phosphorylated histone H2AX; Tirapazamine, 3-amino-1,2,4-benzotriazine-1,3-dioxide.

b

Images such as those shown in Fig. 3, E and F, were compared pixel by pixel. The maximum intensity of γH2AX and Hoechst 33342-positive pixels in each section was assigned the value of 1.0.

c

Mean and SE for n = 10–12 sections.

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