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Table 1

Cellular immune responses to multiple tumor antigens after therapy with vaccine and radiation

TreatmentdAntigena
ConAHSACEA (μg/ml)bp53 (μg/ml)Peptidec
2512.56.251.250.6250.3125CEAp53gp70
Vaccine + Radiation 930 0.8 3.9 2.9 1.7 3.8 3.6 2.6 2,000 320 28,940 
Control 887 1.3 1.1 0.8 1.0 1.2 0.7 0.7 <2.5e <2.5 <2.5 
TreatmentdAntigena
ConAHSACEA (μg/ml)bp53 (μg/ml)Peptidec
2512.56.251.250.6250.3125CEAp53gp70
Vaccine + Radiation 930 0.8 3.9 2.9 1.7 3.8 3.6 2.6 2,000 320 28,940 
Control 887 1.3 1.1 0.8 1.0 1.2 0.7 0.7 <2.5e <2.5 <2.5 
a

For proliferation, antigen concentrations were ConA (2.5 μg/ml), human serum albumin (25 μg/ml), CEA (25–6.25 μg/ml), or p53 (1.25–0.3125 μg/ml). Each value represents the stimulation index of the mean CPM of triplicate samples versus media. SD never exceeded 10%.

b

CEA, carcinoembryonic antigen; ConA, concanavalin A; rV, recombinant vaccinia; TRICOM, triad of costimulatory molecules; rF, recombinant fowlpox; GM-CSF, granulocyte macrophage colony-stimulated factor.

c

For peptide-specific IFN-γ production, concentrations of peptides CEA, p53, and gp70 were 10, 2, and 1 μg/ml, respectively. Each value represents IFN-γ (pg/ml/106 cells/24 h).

d

Three CEA-Tg mice/group were given MC38-CEA+ tumors s.c. Eight days later mice were vaccinated with rV-CEA/TRICOM admixed with rF-GM-CSF. On day 14, tumors were irradiated (8 Gy). On days 15, 22, and 29, mice were boosted with rF-CEA/TRICOM admixed with rF-GM-CSF. Responses from pooled splenic T cells from cured mice were analyzed 6 months following the tumor transplant for CEA protein and p53 MHC-II-peptide-specific proliferation and CEA, p53, and gp70 peptide-specific IFN-γ production. Control mice, normal age-matched CEA-Tg mice.

e

Detection limit was 2.5 pg/ml.

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