American Association for Cancer Research

Table 1

Summary of xenograft studies

Tumors with an average diameter ≥5 mm were scored as positive. Tumors postcastration were scored positive if the tumors maintained size or continued to grow. Confidence intervals (95%) are provided. Tumor invasion into the abdominal cavity was determined at necropsy. Data are from two independent experiments. Western analysis was used to assess in vitro MAPK phosphorylation on cell cultures grown in serum-free medium, and in vivo MAPK phosphorylation on postcastration tumors.

	LNCaP	C4-2	c-Ras	T35S	E37G	Y40C
Tumor take	8/32	22/24	4/40	25/32	16/32	10/32
	(25%)	(92%)	(13%)	(78%)	(50%)	(31%)
	(0.11–0.43)	(0.73–0.99)	(0.04–0.29)	(0.60–0.91)	(0.32–0.68)	(0.16–0.50)
Average time to tumor take	39 days	25 days	40 days	21 days	29 days	39 days
Tumors postcastration	0/4	11/11	2/2	8/13	3/6	4/5
	(0%)	(91%)	(71%)	(69%)	(50%)	(75%)
	(0.0–0.60)	(0.0.72–1.0)	(0.16–1.0)	(0.32–0.86)	(0.12–0.88)	(0.28–0.99)
Invasive tumors	0/4	0/22	0/11	0/25	0/16	5/12
Constitutive in vitro p-MAPK	No	No	No	Yes	Yes	No
Constitutive in vivo p-MAPK postcastration	n/a^a	++	++++	++++	++++	++++

	LNCaP	C4-2	c-Ras	T35S	E37G	Y40C
Tumor take	8/32	22/24	4/40	25/32	16/32	10/32
	(25%)	(92%)	(13%)	(78%)	(50%)	(31%)
	(0.11–0.43)	(0.73–0.99)	(0.04–0.29)	(0.60–0.91)	(0.32–0.68)	(0.16–0.50)
Average time to tumor take	39 days	25 days	40 days	21 days	29 days	39 days
Tumors postcastration	0/4	11/11	2/2	8/13	3/6	4/5
	(0%)	(91%)	(71%)	(69%)	(50%)	(75%)
	(0.0–0.60)	(0.0.72–1.0)	(0.16–1.0)	(0.32–0.86)	(0.12–0.88)	(0.28–0.99)
Invasive tumors	0/4	0/22	0/11	0/25	0/16	5/12
Constitutive in vitro p-MAPK	No	No	No	Yes	Yes	No
Constitutive in vivo p-MAPK postcastration	n/a^a	++	++++	++++	++++	++++

n/a, not applicable.

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