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Table 1

Summary of xenograft studies

Tumors with an average diameter ≥5 mm were scored as positive. Tumors postcastration were scored positive if the tumors maintained size or continued to grow. Confidence intervals (95%) are provided. Tumor invasion into the abdominal cavity was determined at necropsy. Data are from two independent experiments. Western analysis was used to assess in vitro MAPK phosphorylation on cell cultures grown in serum-free medium, and in vivo MAPK phosphorylation on postcastration tumors.

LNCaPC4-2c-RasT35SE37GY40C
Tumor take 8/32 22/24 4/40 25/32 16/32 10/32 
 (25%) (92%) (13%) (78%) (50%) (31%) 
 (0.11–0.43) (0.73–0.99) (0.04–0.29) (0.60–0.91) (0.32–0.68) (0.16–0.50) 
Average time to tumor take 39 days 25 days 40 days 21 days 29 days 39 days 
Tumors postcastration 0/4 11/11 2/2 8/13 3/6 4/5 
 (0%) (91%) (71%) (69%) (50%) (75%) 
 (0.0–0.60) (0.0.72–1.0) (0.16–1.0) (0.32–0.86) (0.12–0.88) (0.28–0.99) 
Invasive tumors 0/4 0/22 0/11 0/25 0/16 5/12 
Constitutive in vitro p-MAPK No No No Yes Yes No 
Constitutive in vivo p-MAPK postcastration n/aa ++ ++++ ++++ ++++ ++++ 
LNCaPC4-2c-RasT35SE37GY40C
Tumor take 8/32 22/24 4/40 25/32 16/32 10/32 
 (25%) (92%) (13%) (78%) (50%) (31%) 
 (0.11–0.43) (0.73–0.99) (0.04–0.29) (0.60–0.91) (0.32–0.68) (0.16–0.50) 
Average time to tumor take 39 days 25 days 40 days 21 days 29 days 39 days 
Tumors postcastration 0/4 11/11 2/2 8/13 3/6 4/5 
 (0%) (91%) (71%) (69%) (50%) (75%) 
 (0.0–0.60) (0.0.72–1.0) (0.16–1.0) (0.32–0.86) (0.12–0.88) (0.28–0.99) 
Invasive tumors 0/4 0/22 0/11 0/25 0/16 5/12 
Constitutive in vitro p-MAPK No No No Yes Yes No 
Constitutive in vivo p-MAPK postcastration n/aa ++ ++++ ++++ ++++ ++++ 
a

n/a, not applicable.

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