Skip to Main Content
Table 3

Normal donor T-cell responses to putative EphA2-derived peptide epitopes

Responder CD4+ or CD8+ T cells were analyzed for reactivity against the HLA-A2+/DR4+ target cell line T2.DR4 pulsed with no peptides, or pulsed with irrelevant or EphA2-derived peptides. T cell reactivity against T2.DR4 cells pulsed with the HLA-A2-presented HIV-nef190–198 epitope served as the CD8+ cell negative control, while HLA-DR4-presented Malarial circumsporozooite (CS)326–345 epitope served as the CD4+ T cell negative control. These control values were subtracted from experimental determinations in order to determine EphA2-specific T cell responder spot numbersa per 100,000 T cells, bA value of “0” reflects a frequency < 1/100,000 T cells. The appropriate HLA-A2 or −DR4 restricted nature of specific T cell recognition of peptides was validated by inclusion of anti-HLA-A2 or -DR4 mAb in replicate ELISPOT wells, respectively, with >90% inhibition of EphA2-specific recognition observed (data not shown). Values significantly (p < 0.05) elevated over T2.DR4+ control peptide values are italicized.

A. HLA-A2-presented EphA2 peptides
Normal donor #CD8+ T-cell response to peptide on T2.DR4a (IFN-γ spots/105 CD8+ T cells)
162391546883961
A2-1 9 0b 31 
A2-2 40 81 14 85 21 
A2-3 14 10 21 
A2-4 11 58 
A2-5 11 14 172 
A2-6 91 76 145 13 
A2-7 132 37 
A2-8 15 165 
Total Responses 5/8 3/8 6/8 6/8 3/8 
A. HLA-A2-presented EphA2 peptides
Normal donor #CD8+ T-cell response to peptide on T2.DR4a (IFN-γ spots/105 CD8+ T cells)
162391546883961
A2-1 9 0b 31 
A2-2 40 81 14 85 21 
A2-3 14 10 21 
A2-4 11 58 
A2-5 11 14 172 
A2-6 91 76 145 13 
A2-7 132 37 
A2-8 15 165 
Total Responses 5/8 3/8 6/8 6/8 3/8 
B. HLA-DR4-presented EphA2 peptides
Normal donor #CD4+ T-cell response to peptide on T2.DR4a (IFN-γ spots/105 CD4+ T cells)
5363663
DR4-1 43 11 21 
DR4-2 38 36 57 
DR4-3 7 14 
DR4-4 
DR4-5 156 41 
DR4-6 121 67 
DR4-7 54 48 72 
Total Responses 3/7 6/7 6/7 
B. HLA-DR4-presented EphA2 peptides
Normal donor #CD4+ T-cell response to peptide on T2.DR4a (IFN-γ spots/105 CD4+ T cells)
5363663
DR4-1 43 11 21 
DR4-2 38 36 57 
DR4-3 7 14 
DR4-4 
DR4-5 156 41 
DR4-6 121 67 
DR4-7 54 48 72 
Total Responses 3/7 6/7 6/7 
Close Modal

or Create an Account

Close Modal
Close Modal