Selection of EphA2 peptides for analysis
A. Selected HLA-A2 presented EphA2 peptides . | . | . | . | . | ||||
---|---|---|---|---|---|---|---|---|
Sequence start amino acid # . | AA sequence of nonamer . | Binding scorea . | Peptide generated by proteasome . | Peptide synthesized for analysis . | ||||
883 | TLADFDPRV | 1084 | Yes | Yes | ||||
546 | VLLLVLAGV | 1006 | Yes | Yes | ||||
550 | VLAGVGFFI | 556 | No | No | ||||
58 | IMNDMPIYM | 138 | No | No | ||||
961 | SLLGLKDQV | 127 | Yes | Yes | ||||
253 | WLVPIGQCL | 98 | No | No | ||||
12 | LLWGCALAA | 71 | No | No | ||||
391 | GLTRTSVTV | 70 | Yes | Yes | ||||
120 | NLYYAESDL | 68 | No | No | ||||
162 | KLNVEERSV | 49 | Yes | Yes |
A. Selected HLA-A2 presented EphA2 peptides . | . | . | . | . | ||||
---|---|---|---|---|---|---|---|---|
Sequence start amino acid # . | AA sequence of nonamer . | Binding scorea . | Peptide generated by proteasome . | Peptide synthesized for analysis . | ||||
883 | TLADFDPRV | 1084 | Yes | Yes | ||||
546 | VLLLVLAGV | 1006 | Yes | Yes | ||||
550 | VLAGVGFFI | 556 | No | No | ||||
58 | IMNDMPIYM | 138 | No | No | ||||
961 | SLLGLKDQV | 127 | Yes | Yes | ||||
253 | WLVPIGQCL | 98 | No | No | ||||
12 | LLWGCALAA | 71 | No | No | ||||
391 | GLTRTSVTV | 70 | Yes | Yes | ||||
120 | NLYYAESDL | 68 | No | No | ||||
162 | KLNVEERSV | 49 | Yes | Yes |
B. Selected HLA-DR4 presented EphA2 peptides . | . | . | . | |||
---|---|---|---|---|---|---|
Sequence start core AA# . | AA sequence of nonamer core . | Binding scorea . | Peptide synthesized for in vitro analysis . | |||
666 | IMGQFSHHN | 577 | 663EAGIMGQFSHHNIIR677 | |||
67 | YSVCNVMSG | 95 | 63PIYMYSVCNVMSG75 | |||
55 | MQNIMNDMP | 39 | 53DLMQNIMNDMPIYMYS68 |
B. Selected HLA-DR4 presented EphA2 peptides . | . | . | . | |||
---|---|---|---|---|---|---|
Sequence start core AA# . | AA sequence of nonamer core . | Binding scorea . | Peptide synthesized for in vitro analysis . | |||
666 | IMGQFSHHN | 577 | 663EAGIMGQFSHHNIIR677 | |||
67 | YSVCNVMSG | 95 | 63PIYMYSVCNVMSG75 | |||
55 | MQNIMNDMP | 39 | 53DLMQNIMNDMPIYMYS68 |
The higher the binding score, the greater the stability of the predicted peptide-MHC complex. Binding scores and qualitative determination of proteasomal processing were predicted using on-line algorithms as described in “Materials and Methods.”