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a

Appendix 3 Information on nine microsatellite markers used as genetic background in humans

Locus designation (location)aSize range (bp)Dye label% of homozygosity in different populationb (Fst)
African AmericanCaucasianHispanicAsian
D3S1358 114–142 5-FAM (blue) 21.4% 19.2% 23.1% 27.6% 
(3p)   (−0.0005) (−0.0009) (0.0014) (0.0035) 
vWA 157–197 5-FAM 20.9% 24.8% 16.7% 16.8% 
(12p12-pter)   (0.0011) (−0.0011) (0.0029) (0.0027) 
FGA 219–267 5-FAM 14.5% 11.9% 14.1% 10.2% 
(4q28)   (0.0004) (−0.0004) (0.0008) (0.0029) 
D8S1179 128–168 JOE (green) 22.0% 24.4% 17.9% 23.5% 
(8)   (−0.0001) (0.0000) (0.0005) (0.0025) 
D21S11 189–243 JOE 14.5% 18.9% 17.5% 18.9% 
(21)   (0.0005) (0.0008) (0.0013) (0.0056) 
D18S51 273–341 JOE 11.0% 12.9% 10.3% 7.7% 
(18q21.3)   (0.0012) (0.0001) (0.0011) (0.0046) 
D5S818 135–171 NED (yellow) 29.5% 29.7% 28.6% 23.0% 
(5q21-31)   (0.0010) (−0.0001) (0.0010) (0.0028) 
D13S317 206–234 NED 31.6% 25.6% 17.5% 29.1% 
(13q22-31)   (0.0029) (−0.0008) (0.0047) (0.0071) 
D7S820 258–294 NED 24.2% 18.3% 20.1% 23.0% 
(7q)   (0.0000) (−0.0005) (0.0010) (0.0039) 
Locus designation (location)aSize range (bp)Dye label% of homozygosity in different populationb (Fst)
African AmericanCaucasianHispanicAsian
D3S1358 114–142 5-FAM (blue) 21.4% 19.2% 23.1% 27.6% 
(3p)   (−0.0005) (−0.0009) (0.0014) (0.0035) 
vWA 157–197 5-FAM 20.9% 24.8% 16.7% 16.8% 
(12p12-pter)   (0.0011) (−0.0011) (0.0029) (0.0027) 
FGA 219–267 5-FAM 14.5% 11.9% 14.1% 10.2% 
(4q28)   (0.0004) (−0.0004) (0.0008) (0.0029) 
D8S1179 128–168 JOE (green) 22.0% 24.4% 17.9% 23.5% 
(8)   (−0.0001) (0.0000) (0.0005) (0.0025) 
D21S11 189–243 JOE 14.5% 18.9% 17.5% 18.9% 
(21)   (0.0005) (0.0008) (0.0013) (0.0056) 
D18S51 273–341 JOE 11.0% 12.9% 10.3% 7.7% 
(18q21.3)   (0.0012) (0.0001) (0.0011) (0.0046) 
D5S818 135–171 NED (yellow) 29.5% 29.7% 28.6% 23.0% 
(5q21-31)   (0.0010) (−0.0001) (0.0010) (0.0028) 
D13S317 206–234 NED 31.6% 25.6% 17.5% 29.1% 
(13q22-31)   (0.0029) (−0.0008) (0.0047) (0.0071) 
D7S820 258–294 NED 24.2% 18.3% 20.1% 23.0% 
(7q)   (0.0000) (−0.0005) (0.0010) (0.0039) 
a

Information on the location, size, and dye label came from the user’s manual of Applied Biosystems AmpFESTER Profiler Plus PCR Amplification kit.

b

Information on percentage of homozygosity and Fst in different populations was summarized from Budowle et al. (44).

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