Skip to Main Content
Table 1

Inhibition of PI3k in DOV13Mp110∗ cells increases paclitaxel-induced apoptosis

Parental DOV13 or DOV13 Mp110∗ cells were treated with 1 mm paclitaxel in the absence or presence of 20 mm LY294002 as described in “Materials and Methods.” Cells were harvested, and the TUNEL/positive cells (apoptotic cells) were measured using an Apo-BrDU kit to label DNA breaks. The data were analyzed by flow cytometry and are presented as % of TUNEL/positive cells in the total cell population. Fold increase was calculated as number of apoptotic cells after LY294002/number of apoptotic cells after 0.2% DMSO treatment cells and number of apoptotic after paclitaxel treatment/number of apoptotic cells in control medium.

TreatmentDOV13 parental cell lineDOV13Mp110∗ cell line
TUNEL/positive cells (%)(Fold increase)TUNEL/positive cells (%)(Fold increase)
Control 1.5  0.9  
0.2% DMSO 2.9  1.5  
LY294002 (20 mm4.9 (1.7) 3.7 (2.5) 
Paclitaxel (1 mm4.7 (3.1) 0.8 (2.9) 
Paclitaxel + LY294002 7.3 (2.5) 3.2 (2.1) 
TreatmentDOV13 parental cell lineDOV13Mp110∗ cell line
TUNEL/positive cells (%)(Fold increase)TUNEL/positive cells (%)(Fold increase)
Control 1.5  0.9  
0.2% DMSO 2.9  1.5  
LY294002 (20 mm4.9 (1.7) 3.7 (2.5) 
Paclitaxel (1 mm4.7 (3.1) 0.8 (2.9) 
Paclitaxel + LY294002 7.3 (2.5) 3.2 (2.1) 
Close Modal

or Create an Account

Close Modal
Close Modal