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Table 1

Overview of EphA2 monoclonal antibodies

Two groups of EphA2 antibodies were identified in this study: EA and EN antibodies. The antibodies were evaluated for their technical applications as measured using WB, IP, and immunofluorescence staining (IF), as well as for their ability to inhibit tumor cell (MDA-MB-231, PC3) growth in soft agar or tubular network formation on Matrigel (MDA-MB-231, MCFEphA2). The asterisk (∗) signifies a differential ability of EA antibodies to recognize EphA2 during WB. EA antibodies generally did not recognize EphA2 under reducing conditions (see text for details).

AntibodyWBIPIFEphA2 ActivationEphA2 DegradationSoft Agar InhibitionTNa Inhibition
EA Group (EA1.1, EA1.2, EA2, EA3, EA4, EA5) ∗ 
EN Group (EN1, EN2) − − − − 
AntibodyWBIPIFEphA2 ActivationEphA2 DegradationSoft Agar InhibitionTNa Inhibition
EA Group (EA1.1, EA1.2, EA2, EA3, EA4, EA5) ∗ 
EN Group (EN1, EN2) − − − − 
a

TN, tubular network formation.

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