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Table 2

Fe ion-induced mutation spectra for early- and late-arising TK1 mutants

The mutant classification was performed by Southern blot analysis using a TK1 cDNA probe and densitometric analysis of TK1 gene dosage.

Mutant classificationTK6 early-arisingWTK1 early-arisingTK6 late-arisingWTK1 late-arising
LOH 42 45 49 49 
Partial deletion/rearrangement 
No detectable alterations 
Total 50 49 50 50 
LOH via deletion (1 silent allele) 40 30 41 26 
LOH via recombination (2 silent alleles) 12 17 
LOH via recombination or nondisjunction (2 silent alleles) 
Total 42 45 49 49 
 χ2 for LOH type,a 1 df, 11.27; P < 0.001  χ2 for LOH type, 2 df, 10.9; P < 0.005  
Mutant classificationTK6 early-arisingWTK1 early-arisingTK6 late-arisingWTK1 late-arising
LOH 42 45 49 49 
Partial deletion/rearrangement 
No detectable alterations 
Total 50 49 50 50 
LOH via deletion (1 silent allele) 40 30 41 26 
LOH via recombination (2 silent alleles) 12 17 
LOH via recombination or nondisjunction (2 silent alleles) 
Total 42 45 49 49 
 χ2 for LOH type,a 1 df, 11.27; P < 0.001  χ2 for LOH type, 2 df, 10.9; P < 0.005  
a

χ2 analysis compares deletion mutants versus nondeletion mutants.

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