In situ hybridization of adult mouse tissues
| Name . | % idena . | Normal adult tissuesb . | . | . | . | . | . | . | . | . | . | . | . | Tumors . | . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Ad . | Br . | H . | I . | Ki-C . | Ki-M . | Li . | Lg . | M . | P . | Sp . | St . | B16 . | HCT . | ||||||||||||
| mTEM1 | 77 | + | + | + | + | − | − | − | + | + | + | − | − | +++ | +++ | ||||||||||||
| mTEM5 | 87 | − | + | + | + | − | − | − | + | + | + | − | − | +++c | +++ | ||||||||||||
| mTEM7 | 81 | − | +++d | − | − | − | − | − | − | − | − | − | − | − | − | ||||||||||||
| mTEM7R | 91 | − | +e | + | + | − | − | + | +++ | +++ | + | + | − | NDf | +++ | ||||||||||||
| mTEM8 | 96 | − | + | + | + | − | − | − | + | + | + | − | − | +++c | +++ | ||||||||||||
| VEGFR | N/A | +++ | ++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ||||||||||||
| Name . | % idena . | Normal adult tissuesb . | . | . | . | . | . | . | . | . | . | . | . | Tumors . | . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Ad . | Br . | H . | I . | Ki-C . | Ki-M . | Li . | Lg . | M . | P . | Sp . | St . | B16 . | HCT . | ||||||||||||
| mTEM1 | 77 | + | + | + | + | − | − | − | + | + | + | − | − | +++ | +++ | ||||||||||||
| mTEM5 | 87 | − | + | + | + | − | − | − | + | + | + | − | − | +++c | +++ | ||||||||||||
| mTEM7 | 81 | − | +++d | − | − | − | − | − | − | − | − | − | − | − | − | ||||||||||||
| mTEM7R | 91 | − | +e | + | + | − | − | + | +++ | +++ | + | + | − | NDf | +++ | ||||||||||||
| mTEM8 | 96 | − | + | + | + | − | − | − | + | + | + | − | − | +++c | +++ | ||||||||||||
| VEGFR | N/A | +++ | ++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ||||||||||||
The percentage of amino acid identity to homologous human TEMs.
−, no endothelial cell staining detected; +, weak positive staining of endothelial cells; ++, moderate staining of endothelial cells; +++, strong staining of endothelial cells. Ad, adrenal gland; B, brain; H, heart; I, intestine; Ki-C, kidney cortex; Ki-M, kidney medulla; Li, liver; Lg, lung; M, skeletal muscle; P, pancreas; Sp, spleen; St, stomach; HCT, HCT116 human colon carcinoma xenograft; B16, B16 mouse melanoma tumor.
Endothelial cells were strongly positive; some B16 tumor cells were also weakly positive.
Strong staining localized to the Purkinje cells of the cerebellum and some neuronal cells.
Weak staining localized primarily to Purkinje cells.
ND, not determined.