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Table 1

Oligonucleotide primers used for amplification of the entire NAT1 gene and of smaller fragments, enabling reliable detection of point mutations

NameaBinding positionsFragment length (bp)Sequence
N1-1F −415–−394 1540 5′-GAA ATT GAG TGG GTC AGG TAC C 
N1-5R 1125–1106  5′-TTC CAA GAT AAC CAC AGG CC 
N1-IR −7–−39 409 (with N1-1F) 5′-CTA AGC AAG GAA AAC AAA ACG AAA GCA AAT AAT 
N1-5F 691–710 435 5′-GTT CAC TGT TTG GTG GGC TT 
N1-IIR 1125–1096  5′-TTC CAA GAT AAC CAC AGG CCA TCT TTA GAA 
N1-6R 1108–1088 418 (with N1-5F) 5′-GCC ATC TTT AAA ATA CAT TTA 
N1-7R 1108–1088  5′-GCC ATC TTT AAA ATA CAT TTT 
N1-4F 344–364 247 5′-ATG GCA GGA ACT ACA TTG TCG 
N1-IIIR 561–590  5′-CGA GGC TTA AGA GTA AAG GAG TAG ATG TC
N1-8F −16–4 878 5′-TCC TTG CTT AGG GGA TCA TG 
N1-8R 861–840  5′-AAA TCT ATC ACC ATG TTT GGG C 
NameaBinding positionsFragment length (bp)Sequence
N1-1F −415–−394 1540 5′-GAA ATT GAG TGG GTC AGG TAC C 
N1-5R 1125–1106  5′-TTC CAA GAT AAC CAC AGG CC 
N1-IR −7–−39 409 (with N1-1F) 5′-CTA AGC AAG GAA AAC AAA ACG AAA GCA AAT AAT 
N1-5F 691–710 435 5′-GTT CAC TGT TTG GTG GGC TT 
N1-IIR 1125–1096  5′-TTC CAA GAT AAC CAC AGG CCA TCT TTA GAA 
N1-6R 1108–1088 418 (with N1-5F) 5′-GCC ATC TTT AAA ATA CAT TTA 
N1-7R 1108–1088  5′-GCC ATC TTT AAA ATA CAT TTT 
N1-4F 344–364 247 5′-ATG GCA GGA ACT ACA TTG TCG 
N1-IIIR 561–590  5′-CGA GGC TTA AGA GTA AAG GAG TAG ATG TC
N1-8F −16–4 878 5′-TCC TTG CTT AGG GGA TCA TG 
N1-8R 861–840  5′-AAA TCT ATC ACC ATG TTT GGG C 
a

F, forward primer; R, reverse primer; roman numerals, designed primers; mismatch base is indicated in italic bold.

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