Skip to Main Content
Table 1

DNA damage-induced DR5 up-regulation and synergistic cytotoxicity by combination treatment with TRAIL and genotoxic agents in human glioma cell lines

Cells were treated with drugs for 16 h for RNA preparation and 24 h for cytotoxicity assays.

Cell lineDR5 up-regulationSynergistic cytotoxicitya
CDDP (μg/ml)VP16 (μg/ml)BCNU (μM)CDDPb + TRAILcVP16b + TRAILcBCNUb + TRAILc
Responders       
U87MG + (4) + (16) ± (100) − 
A1207 + (10) + (16) + (200) 
U178MG + (4) + (16) + (100) − 
LN229 + (10) + (16) − (100) − 
U251MG + (4) + (8) + (200) 
LN428 + (10) + (64) − (300) ± 
Intermediate-responder       
T98G ± (10) − (128) − (200) ± − − 
Nonresponders       
U373MG − (40) − (128) ± (100) − − − 
LNZ308 − (40) − (128) − (100) − − − 
Cell lineDR5 up-regulationSynergistic cytotoxicitya
CDDP (μg/ml)VP16 (μg/ml)BCNU (μM)CDDPb + TRAILcVP16b + TRAILcBCNUb + TRAILc
Responders       
U87MG + (4) + (16) ± (100) − 
A1207 + (10) + (16) + (200) 
U178MG + (4) + (16) + (100) − 
LN229 + (10) + (16) − (100) − 
U251MG + (4) + (8) + (200) 
LN428 + (10) + (64) − (300) ± 
Intermediate-responder       
T98G ± (10) − (128) − (200) ± − − 
Nonresponders       
U373MG − (40) − (128) ± (100) − − − 
LNZ308 − (40) − (128) − (100) − − − 
a

More than 20% increase in the percentage of growth inhibition than simple addition of the percentage of growth inhibition of the two drugs was evaluated as +, more than 10% increase as ±.

b

At the same concentrations used in the treatment for DR5 up-regulation. Single treatment with each of these drugs at the same concentrations was ineffective in all cell lines.

c

TRAIL (0.1 μg/ml) was used for all combination treatments. Single treatment with 10 μg/ml TRAIL was ineffective in all cell lines.

Close Modal

or Create an Account

Close Modal
Close Modal