Skip to Main Content
Table 1

Correlation between anti-CD20-and BCR-mediated apoptosis

The indicated cell lines (BL60-2, sIgM-sensitive subclone of BL60;BL41-s, sIgM-sensitive subclone, BL41-r, sIgM-resistant subclone of BL41) were incubated in the presence of immobilized IDEC-C2B8 or soluble anti-sIgM F(ab′)2 fragments. After 20 h, cells were stained with acridine orange, and the number of apoptotic cells per 100 cells was determined by fluorescence microscopy. Cell surface antigen expression was determined by immunofluorescence staining. The MFI of CD20 or sIgM staining is shown.

Cell lineAntigen expression (MFI)Apoptosis sensitivity
CD20IgMAnti-CD20Anti-IgM
BL60.2 265.2 11.2 ++++ ++++ 
BL41-s 435.7 39.2 ++ ++ 
BL41-r 114.1 22.2 − − 
Daudi 90.4 29.2 − − 
BJAB 309.4 12.4 − − 
++++ >40%     
+++ 30–40%     
++ 20–30% Apoptotic cells    
+ 10–20%     
− 0–10%     
Cell lineAntigen expression (MFI)Apoptosis sensitivity
CD20IgMAnti-CD20Anti-IgM
BL60.2 265.2 11.2 ++++ ++++ 
BL41-s 435.7 39.2 ++ ++ 
BL41-r 114.1 22.2 − − 
Daudi 90.4 29.2 − − 
BJAB 309.4 12.4 − − 
++++ >40%     
+++ 30–40%     
++ 20–30% Apoptotic cells    
+ 10–20%     
− 0–10%     
Close Modal

or Create an Account

Close Modal
Close Modal