Table 1
Correlation between anti-CD20-and BCR-mediated apoptosis
The indicated cell lines (BL60-2, sIgM-sensitive subclone of BL60;BL41-s, sIgM-sensitive subclone, BL41-r, sIgM-resistant subclone of BL41) were incubated in the presence of immobilized IDEC-C2B8 or soluble anti-sIgM F(ab′)2 fragments. After 20 h, cells were stained with acridine orange, and the number of apoptotic cells per 100 cells was determined by fluorescence microscopy. Cell surface antigen expression was determined by immunofluorescence staining. The MFI of CD20 or sIgM staining is shown.
| Cell line | Antigen expression (MFI) | Apoptosis sensitivity | ||||
|---|---|---|---|---|---|---|
| CD20 | IgM | Anti-CD20 | Anti-IgM | |||
| BL60.2 | 265.2 | 11.2 | ++++ | ++++ | ||
| BL41-s | 435.7 | 39.2 | ++ | ++ | ||
| BL41-r | 114.1 | 22.2 | − | − | ||
| Daudi | 90.4 | 29.2 | − | − | ||
| BJAB | 309.4 | 12.4 | − | − | ||
++++ >40% | ||||||
| +++ 30–40% | ||||||
| ++ 20–30% | Apoptotic cells | |||||
| + 10–20% | ||||||
| − 0–10% | ||||||
| Cell line | Antigen expression (MFI) | Apoptosis sensitivity | ||||
|---|---|---|---|---|---|---|
| CD20 | IgM | Anti-CD20 | Anti-IgM | |||
| BL60.2 | 265.2 | 11.2 | ++++ | ++++ | ||
| BL41-s | 435.7 | 39.2 | ++ | ++ | ||
| BL41-r | 114.1 | 22.2 | − | − | ||
| Daudi | 90.4 | 29.2 | − | − | ||
| BJAB | 309.4 | 12.4 | − | − | ||
| ++++ >40% | ||||||
| +++ 30–40% | ||||||
| ++ 20–30% | Apoptotic cells | |||||
| + 10–20% | ||||||
| − 0–10% | ||||||